Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.8.2.18 extracted from

  • Singer, S.S.; Bruns, L.
    Enzymatic sulfation of steroids. XI. The extensive purification and some properties of hepatic sulfotransferase III from female rats (1980), Can. J. Biochem., 58, 660-666.
    View publication on PubMed
Search for more literature for 2.8.2.18

Inhibitors

Inhibitors Comment Organism Structure
adenosine 3',5'-bisphosphate product inhibition Rattus norvegicus
Cd2+ 5 mM, strong inhibition Rattus norvegicus
corticosterone cortisol as substrate, strong Rattus norvegicus
Cortisol 21-sulfate product inhibition Rattus norvegicus
cortisone cortisol as substrate, weak, 0.012 mM Rattus norvegicus
dehydroepiandrosterone cortisol as substrate; strong Rattus norvegicus
deoxycorticosterone cortisol as substrate, strong Rattus norvegicus
dexamethasone cortisol as substrate; weak Rattus norvegicus
diethylstilbestrol cortisol as substrate; strong Rattus norvegicus
estradiol-17beta 44% inhibition at 0.012 mM; cortisol as substrate Rattus norvegicus
progesterone cortisol as substrate; strong Rattus norvegicus
testosterone cortisol as substrate, strong Rattus norvegicus
tetrahydrocortisol not Rattus norvegicus
Zn2+ 5 mM, strong inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00648
-
 cortisol pH 6.8 Rattus norvegicus
0.00678
-
 3'-phosphoadenylylsulfate pH 6.8 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activation Rattus norvegicus
Ba2+ 5 mM Rattus norvegicus
Ba2+ in decreasing order of efficiency: Cr2+, Mg2+, Ba2+, Mn2+, Ni2+, Ca2+, Co2+ Rattus norvegicus
Ca2+ activation Rattus norvegicus
Ca2+ 5 mM Rattus norvegicus
Ca2+ in decreasing order of efficiency: Cr2+, Mg2+, Ba2+, Mn2+, Ni2+, Ca2+, Co2+ Rattus norvegicus
Co2+ activation Rattus norvegicus
Co2+ 5 mM Rattus norvegicus
Co2+ in decreasing order of efficiency: Cr2+, Mg2+, Ba2+, Mn2+, Ni2+, Ca2+, Co2+ Rattus norvegicus
Cr2+ activation Rattus norvegicus
Cr2+ 5 mM Rattus norvegicus
Cr2+ in decreasing order of efficiency: Cr2+, Mg2+, Ba2+, Mn2+, Ni2+, Ca2+, Co2+ Rattus norvegicus
Fe3+ not Rattus norvegicus
Mg2+ activation Rattus norvegicus
Mg2+ 5 mM Rattus norvegicus
Mg2+ in decreasing order of efficiency: Cr2+, Mg2+, Ba2+, Mn2+, Ni2+, Ca2+, Co2+ Rattus norvegicus
Mn2+ activation Rattus norvegicus
Mn2+ 5 mM Rattus norvegicus
Mn2+ in decreasing order of efficiency: Cr2+, Mg2+, Ba2+, Mn2+, Ni2+, Ca2+, Co2+ Rattus norvegicus
Ni2+ activation Rattus norvegicus
Ni2+ 5 mM Rattus norvegicus
Ni2+ in decreasing order of efficiency: Cr2+, Mg2+, Ba2+, Mn2+, Ni2+, Ca2+, Co2+ Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + cortisol Rattus norvegicus
-
 adenosine 3',5'-bisphosphate + cortisol 21-sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 female Sprague-Dawley CD rats
-

Purification (Commentary)

Purification (Comment) Organism
sulfotransferase III Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
3'-phosphoadenylyl sulfate + cortisol = adenosine 3',5'-bisphosphate + cortisol 21-sulfate mechanism Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.016
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, at least 3 weeks Rattus norvegicus
0-4°C, in crude extracts, 3 days Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + cortisol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + cortisol 21-sulfate
-
 ?
3'-phosphoadenylylsulfate + cortisol i.e. PAPS, glucocorticoids are preferred substrates Rattus norvegicus adenosine 3',5'-bisphosphate + cortisol 21-sulfate
-
 ?
3'-phosphoadenylylsulfate + deoxycorticosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + estradiol-17beta
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + testosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 cortisol as substrate Rattus norvegicus