Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2',5'-ADP | - |
Synechococcus sp. | |
3',5'-ADP | product inhibition | Synechococcus sp. | |
5'-ADP | no inhibition | Synechococcus sp. | |
5'-AMP | no inhibition | Synechococcus sp. | |
Mg2+ | above 50 mM, activation at 5-10 mM | Synechococcus sp. | |
additional information | no inhibition by 2'-AMP, 3'-AMP, cAMP; no inhibition by cysteic acid, cysteine or methionine | Synechococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
3'-phosphoadenylylsulfate | pH 8.0 | Synechococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 20% activation at 5-10 mM, severe inhibition above 50 mM | Synechococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
gel filtration | Synechococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + a thiol | Synechococcus sp. | involved in assimilatory sulfate reduction | adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate | - |
? | |
3'-phosphoadenylylsulfate + a thiol | Synechococcus sp. 6301 | involved in assimilatory sulfate reduction | adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechococcus sp. | - |
cyanobacterium | - |
Synechococcus sp. 6301 | - |
cyanobacterium | - |
Purification (Comment) | Organism |
---|---|
partial | Synechococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + a thiol | i.e. 3'-phosphoadenosine 5'-phosphosulfate or PAPS, specific for PAPS | Synechococcus sp. | adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate | i.e. 3',5'-ADP | ? | |
3'-phosphoadenylylsulfate + a thiol | involved in assimilatory sulfate reduction | Synechococcus sp. | adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate | - |
? | |
3'-phosphoadenylylsulfate + a thiol | i.e. 3'-phosphoadenosine 5'-phosphosulfate or PAPS, specific for PAPS | Synechococcus sp. 6301 | adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate | i.e. 3',5'-ADP | ? | |
3'-phosphoadenylylsulfate + a thiol | involved in assimilatory sulfate reduction | Synechococcus sp. 6301 | adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate | - |
? | |
3'-phosphoadenylylsulfate + dithioerythritol | - |
Synechococcus sp. | ? | - |
? | |
3'-phosphoadenylylsulfate + dithioerythritol | - |
Synechococcus sp. 6301 | ? | - |
? | |
additional information | specificity | Synechococcus sp. | ? | - |
? | |
additional information | adenosine 5'-phosphosulfate (APS) is no substrate | Synechococcus sp. | ? | - |
? | |
additional information | specificity | Synechococcus sp. 6301 | ? | - |
? | |
additional information | adenosine 5'-phosphosulfate (APS) is no substrate | Synechococcus sp. 6301 | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Synechococcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | about 60% of maximal activity at pH 7.0 and about half-maximal activity at pH 10.0 | Synechococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | requirement | Synechococcus sp. |