Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.8.2.16 extracted from

  • Schmidt, A.; Christen, U.
    A factor-dependent sulfotransferase specific for 3'-phosphoadenosine-5'-phosphosulfate (PAPS) in the cyanobacterium Synechococus 6301 (1978), Planta, 140, 239-244.
    View publication on PubMed
Search for more literature for 2.8.2.16

Inhibitors

Inhibitors Comment Organism Structure
2',5'-ADP
-
 Synechococcus sp.
3',5'-ADP product inhibition Synechococcus sp.
5'-ADP no inhibition Synechococcus sp.
5'-AMP no inhibition Synechococcus sp.
Mg2+ above 50 mM, activation at 5-10 mM Synechococcus sp.
additional information no inhibition by 2'-AMP, 3'-AMP, cAMP; no inhibition by cysteic acid, cysteine or methionine Synechococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 3'-phosphoadenylylsulfate pH 8.0 Synechococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 20% activation at 5-10 mM, severe inhibition above 50 mM Synechococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 gel filtration Synechococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + a thiol Synechococcus sp. involved in assimilatory sulfate reduction adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate
-
 ?
3'-phosphoadenylylsulfate + a thiol Synechococcus sp. 6301 involved in assimilatory sulfate reduction adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Synechococcus sp.
-
 cyanobacterium
-
Synechococcus sp. 6301
-
 cyanobacterium
-

Purification (Commentary)

Purification (Comment) Organism
partial Synechococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + a thiol i.e. 3'-phosphoadenosine 5'-phosphosulfate or PAPS, specific for PAPS Synechococcus sp. adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate i.e. 3',5'-ADP ?
3'-phosphoadenylylsulfate + a thiol involved in assimilatory sulfate reduction Synechococcus sp. adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate
-
 ?
3'-phosphoadenylylsulfate + a thiol i.e. 3'-phosphoadenosine 5'-phosphosulfate or PAPS, specific for PAPS Synechococcus sp. 6301 adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate i.e. 3',5'-ADP ?
3'-phosphoadenylylsulfate + a thiol involved in assimilatory sulfate reduction Synechococcus sp. 6301 adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate
-
 ?
3'-phosphoadenylylsulfate + dithioerythritol
-
 Synechococcus sp. ?
-
 ?
3'-phosphoadenylylsulfate + dithioerythritol
-
 Synechococcus sp. 6301 ?
-
 ?
additional information specificity Synechococcus sp. ?
-
 ?
additional information adenosine 5'-phosphosulfate (APS) is no substrate Synechococcus sp. ?
-
 ?
additional information specificity Synechococcus sp. 6301 ?
-
 ?
additional information adenosine 5'-phosphosulfate (APS) is no substrate Synechococcus sp. 6301 ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Synechococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 10 about 60% of maximal activity at pH 7.0 and about half-maximal activity at pH 10.0 Synechococcus sp.

Cofactor

Cofactor Comment Organism Structure
thioredoxin requirement Synechococcus sp.