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Literature summary for 2.8.2.14 extracted from
- The critical role of His48 in mouse cytosolic sulfotransferase SULT2A8 for the 7alpha-hydroxyl sulfation of bile acids (2018), Biosci. Biotechnol. Biochem., 82, 1359-1365 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Sult2a8, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia ocli strain BL21 | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H48N | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Mus musculus |
| H48T | site-directed mutagenesis, inactive mutant | Mus musculus |
| L99H | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
cholic acid | pH 6.3, 37°C, recombinant wild-type enzyme | Mus musculus |  |
| 0.1859 | - |
cholic acid | pH 6.3, 37°C, recombinant mutant H48N | Mus musculus | |
| 0.3935 | - |
cholic acid | pH 6.3, 37°C, recombinant mutant L99H | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + chenodeoxycholic acid | Mus musculus | - |
adenosine 3',5'-bisphosphate + chenodeoxycholate 3-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + cholic acid | Mus musculus | high activity | adenosine 3',5'-bisphosphate + cholate 3-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + glycocholic acid | Mus musculus | high activity | adenosine 3',5'-bisphosphate + glycocholate 3-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + taurochenodeoxycholic acid | Mus musculus | - |
adenosine 3',5'-bisphosphate + taurochenodeoxycholate 3-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + taurocholic acid | Mus musculus | best substrate | adenosine 3',5'-bisphosphate + taurocholate 3-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + ursodeoxycholic acid | Mus musculus | - |
adenosine 3',5'-bisphosphate + ursodeoxycholate 3-sulfate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q8BGL3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged wild-type and mutant enzymes from Escherichia ocli strain BL21 by glutathione affinity chromatography, the tag is cleaved off by bovine thrombin | Mus musculus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 3'-phosphoadenylyl sulfate + glycolithocholate = adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate | catalytic mechanism of SULT2A8 | Mus musculus | |
| 3'-phosphoadenylyl sulfate + taurolithocholate = adenosine 3',5'-bisphosphate + taurolithocholate sulfate | catalytic mechanism of SULT2A8 | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.31 | - |
pH 6.3, 37°C, purified recombinant wild-type enzyme, substrate ursodeoxycholic acid | Mus musculus |
| 7.43 | - |
pH 6.3, 37°C, purified recombinant enzyme mutant H48N, substrate cholic acid | Mus musculus |
| 8.72 | - |
pH 6.3, 37°C, purified recombinant enzyme mutant H48N, substrate glycocholic acid | Mus musculus |
| 11.6 | - |
pH 6.3, 37°C, purified recombinant enzyme mutant L99H, substrate glycocholic acid | Mus musculus |
| 26.3 | - |
pH 6.3, 37°C, purified recombinant enzyme mutant L99H, substrate cholic acid | Mus musculus |
| 38.1 | - |
pH 6.3, 37°C, purified recombinant wild-type enzyme, substrate chenodeoxycholic acid | Mus musculus |
| 70.7 | - |
pH 6.3, 37°C, purified recombinant wild-type enzyme, substrate cholic acid | Mus musculus |
| 74.1 | - |
pH 6.3, 37°C, purified recombinant wild-type enzyme, substrate taurochenodeoxycholic acid | Mus musculus |
| 89.1 | - |
pH 6.3, 37°C, purified recombinant wild-type enzyme, substrate glycocholic acid | Mus musculus |
| 89.5 | - |
pH 6.3, 37°C, purified recombinant wild-type enzyme, substrate taurocholic acid | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + chenodeoxycholic acid | - |
Mus musculus | adenosine 3',5'-bisphosphate + chenodeoxycholate 3-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + cholic acid | high activity | Mus musculus | adenosine 3',5'-bisphosphate + cholate 3-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + glycocholic acid | high activity | Mus musculus | adenosine 3',5'-bisphosphate + glycocholate 3-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + taurochenodeoxycholic acid | - |
Mus musculus | adenosine 3',5'-bisphosphate + taurochenodeoxycholate 3-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + taurocholic acid | best substrate | Mus musculus | adenosine 3',5'-bisphosphate + taurocholate 3-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + ursodeoxycholic acid | - |
Mus musculus | adenosine 3',5'-bisphosphate + ursodeoxycholate 3-sulfate | - |
? | |
| additional information | mouse SULT2A8 displays 7alpha-hydroxyl bile acid-preferring activity and is likely responsible for the production of bile acid-7-O-sulfates. SULT2A8 exhibits the highest substrate affinity and catalytic activity toward cholic acid among other 7alpha-hydroxyl bile acids. No activity with deoxycholic acid, pregnenolone, and dehydroepiandrosterone | Mus musculus | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32295, sequence calculation, x * 33000, recombinant detagged enzyme, SDS-PAGE | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sulfotransferase | - |
Mus musculus |
| SULT2A8 | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
assay at | Mus musculus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | substitutions of the two amino acids His48 and Leu99, which might have occurred during the evolution of the mouse SULT2A8 gene, endow mouse SULT2A8 the capacity to catalyze the 7-O-sulfation of bile acids | Mus musculus |
| additional information | critical role of His48 in mouse cytosolic sulfotransferase SULT2A8 for the 7alpha-hydroxyl sulfation of bile acids. SULT2A8 lacks a conservative catalytic His residue at position 99th. Residues, His48 and Leu99 are unique to the mouse SULT2A8, but not other SULTs in general. They are essential for the enzyme's 7-O-sulfating activity toward bile acids and the strict substrate specificity toward 7alpha-hydroxyl bile acids | Mus musculus |
| physiological function | members of the cytosolic sulfotransferase (SULT) SULT2A subfamily are known to be critically involved in the homeostasis of steroids and bile acids. SULT2A8, a 7alpha-hydroxyl bile acid-preferring mouse SULT, has been identified as the major enzyme responsible for the mouse-specific 7-O-sulfation of bile acids | Mus musculus |
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