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Literature summary for 2.8.2.14 extracted from

  • Huang, J.; Bathena, S.P.; Tong, J.; Roth, M.; Hagenbuch, B.; Alnouti, Y.
    Kinetic analysis of bile acid sulfation by stably expressed human sulfotransferase 2A1 (SULT2A1) (2010), Xenobiotica, 40, 184-194.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293 cells Homo sapiens
expressed in HEK293 cells Homo sapiens

General Stability

General Stability Organism
SULT2A1 activity is consistently higher using NaH2PO4 buffer in a pH range from 5.5 to 6.5 Homo sapiens
SULT2A1 activity is consistently highest using NaH2PO4 buffer in a pH range from 5.5 to 6.5 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00186
-
taurine conjugated lithocholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
0.00192
-
glycine conjugated lithocholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
0.00202
-
lithocholic acid apparent value, at pH 5.5 and 7.4, at 37°C Homo sapiens
0.00202
-
lithocholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
0.00383
-
dehydroepiandrosterone apparent value, at pH 5.5 and 7.4, at 37°C Homo sapiens
0.00383
-
dehydroepiandrosterone apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
0.00886
-
deoxycholic acid apparent value, at pH 5.5 and 7.4, at 37°C Homo sapiens
0.00886
-
deoxycholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
0.00993
-
taurine conjugated ursodeoxycholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
0.01286
-
Ursodeoxycholic acid apparent value, at pH 5.5 and 7.4, at 37°C Homo sapiens
0.01286
-
ursodeoxycholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
0.01566
-
chenodeoxycholic acid apparent value, at pH 5.5 and 7.4, at 37°C Homo sapiens
0.01566
-
chenodeoxycholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
0.01836
-
taurine conjugated deoxycholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
0.04509
-
taurine conjugated chenodeoxycholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
0.04899
-
glycine conjugated chenodeoxycholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
0.04957
-
glycine conjugated ursodeoxycholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
0.05459
-
glycine conjugated deoxycholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the addition of 2 mM MgCl2 to the reaction causes a 20% increase in sulfation activity, whereas higher concentrations of Mg2+ inhibit enzyme activity Homo sapiens
Mg2+ the presence of MgCl2 is not required for the SULT2A1 enzyme activity. However, the addition of 2 mM MgCl2 to the reaction causes a 20% increase in sulfation activity, whereas higher concentrations of Mg2+ inhibit SULT2A1 activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33780
-
x * 34000, SDS-PAGE, x * 33780, calculated from amino acid sequence Homo sapiens
34000
-
x * 34000, SDS-PAGE, x * 33780, calculated from amino acid sequence Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + chenodeoxycholic acid Homo sapiens
-
adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylylsulfate + cholic acid Homo sapiens lowest affinity to sulfation by SULT2A1 adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone Homo sapiens
-
adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylylsulfate + deoxycholic acid Homo sapiens
-
adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylylsulfate + lithocholic acid Homo sapiens highest affinity to sulfation by SULT2A1 adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylylsulfate + ursodeoxycholic acid Homo sapiens
-
adenosine 3',5'-bisphosphate + ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens Q06520
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl-sulfate + chenodeoxycholate
-
Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylyl-sulfate + dehydroepiandrosterone
-
Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylyl-sulfate + deoxycholate
-
Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylyl-sulfate + glycine conjugated chenodeoxycholate
-
Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylyl-sulfate + glycine conjugated deoxycholate
-
Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylyl-sulfate + glycine conjugated lithocholate
-
Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylyl-sulfate + glycine conjugated ursodeoxycholate
-
Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylyl-sulfate + lithocholate highest affinity substrate Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylyl-sulfate + taurine conjugated chenodeoxycholate
-
Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylyl-sulfate + taurine conjugated deoxycholate
-
Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylyl-sulfate + taurine conjugated lithocholate
-
Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylyl-sulfate + taurine conjugated ursodeoxycholate
-
Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylyl-sulfate + ursodeoxycholate
-
Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylylsulfate + chenodeoxycholic acid
-
Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylylsulfate + cholic acid lowest affinity to sulfation by SULT2A1 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylylsulfate + deoxycholic acid
-
Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylylsulfate + lithocholic acid highest affinity to sulfation by SULT2A1 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylylsulfate + ursodeoxycholic acid
-
Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
additional information no activity with cholate, glycine conjugated cholate and taurine conjugated cholate Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE, x * 33780, calculated from amino acid sequence Homo sapiens

Synonyms

Synonyms Comment Organism
dehydroepiandrosterone-SULT
-
Homo sapiens
DHEA-SULT
-
Homo sapiens
hydroxysteroid sulfotransferase
-
Homo sapiens
sulfotransferase 2A1
-
Homo sapiens
SULT2A1
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4 9 a more than 10fold increase in enzyme activity is observed with increasing the pH from 4.0 to 5.5, while further increase in pH in 10 mM Tris-HCl buffer is associated with a decline in the enzyme activity Homo sapiens
4 9 more than a 10fold increase in SULT2A1 activity is observed with increasing the pH from 4.0 to 5.5, while a further increase in pH in 10 mM Tris-HCl buffer (up to pH 9-0) is associated with a 50% decline in the optimum enzyme activity Homo sapiens