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Literature summary for 2.8.2.11 extracted from

  • Nagai, K.I.; Tadano-Aritomi, K.; Niimura, Y.; Ishizuka, I.
    Development and application of a system for seminolipid metabolism using mouse seminiferous tubules (2010), Glycoconj. J., 27, 181-187.
    View publication on PubMed

Application

Application Comment Organism
diagnostics the newly developed system could provide useful basic data for the further analysis of seminolipid metabolism Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Mus musculus
-
seminiferous tubule
-
Mus musculus
-
testis
-
Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000000011
-
crude CST preparation, 44°C, 10 min + 32°C, 60 min Mus musculus
0.000000016
-
seminiferous tubule, 44°C, 10 min + 32°C, 60 min Mus musculus
0.000000022
-
crude CST preparation, 37°C, 70 min Mus musculus
0.000000063
-
crude CST prepared from mouse kidney homogenates, 37°C, 70 min Mus musculus
0.000000093
-
seminiferous tubule, 37°C, 70 min Mus musculus
0.000000125
-
crude CST preparation, 32°C, 70 min Mus musculus
0.000000232
-
crude CST prepared from mouse kidney homogenates, 32°C, 70 min Mus musculus
0.000000238
-
crude CST prepared from mouse kidney homogenates, untreated Mus musculus
0.00000025
-
seminiferous tubule, 32°C, 70 min Mus musculus
0.000000343
-
crude CST preparation, untreated Mus musculus
0.000000365
-
seminiferous tubule, untreated Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + galactosylceramide
-
Mus musculus adenosine 3',5'-bisphosphate + galactosylceramide sulfate
-
?

Synonyms

Synonyms Comment Organism
CST
-
Mus musculus
galactocerebroside sulfotransferase
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
activity assay Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
activity assay Mus musculus

Expression

Organism Comment Expression
Mus musculus the activity of galactocerebroside sulfotransferase decreases in a temperature-dependent manner down