Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus | |
vitamin K1 | with phosphate, activates purified preparation | Mus musculus |
General Stability | Organism |
---|---|
ethylene glycol, 2-mercaptoethanol, 10 mM ATP and a mixture of phosphatidylcholine and phosphatidylethanolamine ratio 1:1 does not improve stability at -80°C | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
3'-phosphoadenylylsulfate | pH 7.0, 37°C | Mus musculus | |
0.026 | - |
cerebroside | pH 7.0, 37°C | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
gel filtration | Mus musculus |
31000 | - |
1 * 31000 SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
galactocerebroside + 3'-phosphoadenylylsulfate | Mus musculus | enzyme catalyses the biosynthesis of sulfatides, sulfatides are important components of myelin | sulfogalactoglycerolipid + adenosine 3',5'-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Mus musculus |
Storage Stability | Organism |
---|---|
-80°C, 35% loss of activity after 7 days | Mus musculus |
-80°C, addition of vitamin K + phosphate, stable for 14 days | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + a galactosylceramide | - |
Mus musculus | adenosine 3',5'-bisphosphate + a galactosylceramidesulfate | - |
? | |
3'-phosphoadenylyl sulfate + cerebroside | - |
Mus musculus | adenosine 3',5'-bisphosphate + cerebroside 3-sulfate | - |
? | |
galactocerebroside + 3'-phosphoadenylylsulfate | - |
Mus musculus | galactocerebroside 3-sulfate + adenosine 3',5'-bisphosphate | - |
? | |
galactocerebroside + 3'-phosphoadenylylsulfate | enzyme catalyses the biosynthesis of sulfatides, sulfatides are important components of myelin | Mus musculus | sulfogalactoglycerolipid + adenosine 3',5'-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 31000 SDS-PAGE | Mus musculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
10 min, complete loss of activity | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Mus musculus |