Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoA | CoA may be the competitive inhibitor against PAP in Cypridina sulfotransferase(s) and can also act as a sulfate acceptor | Vargula hilgendorfii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Vargula hilgendorfii | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + Renilla luciferin | Vargula hilgendorfii | - |
adenosine 3',5'-bisphosphate + luciferyl sulfate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vargula hilgendorfii | - |
i.e. Vargula hilgendorfii, collected at Chita (Aichi, Japan) on August 4, 2011, and Naruto (Tokushima, Japan) on August 24, 2017 | - |
Purification (Comment) | Organism |
---|---|
partially by gel filtration and ultrafiltration | Vargula hilgendorfii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-AMP + luciferyl sulfate | - |
Vargula hilgendorfii | 3'-AMP sulfate + luciferin | - |
? | |
3'-phosphoadenylyl sulfate + Renilla luciferin | - |
Vargula hilgendorfii | adenosine 3',5'-bisphosphate + luciferyl sulfate | - |
r | |
5'-AMP + luciferyl sulfate | - |
Vargula hilgendorfii | 5'-AMP sulfate + luciferin | - |
? | |
CoA + luciferyl sulfate | - |
Vargula hilgendorfii | CoA sulfate + luciferin | - |
? | |
additional information | CoA can act as a sulfate acceptor in the sulfotransferase reaction, and can also competitively inhibit the sulfotransferase. Sulfotransferase(s) in Cypridina specimens can efficiently use CoA, 5'-AMP and 3'-AMP as a sulfate acceptor from Cypridina luciferyl sulfate and produce Cypridina luciferin, similar to the case with PAP. Mass spectral analyses of the sulfated products of 5'-AMP, 3'-AMP, and CoA | Vargula hilgendorfii | ? | - |
- |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 25 | assay at | Vargula hilgendorfii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | assay at | Vargula hilgendorfii |
General Information | Comment | Organism |
---|---|---|
physiological function | in the luminous ostracod Cypridina (presently Vargula) hilgendorfii, Cypridina luciferyl sulfate (3-enol sulfate of Cypridina luciferin) is converted to Cypridina luciferin by a sulfotransferase with 3'-phosphoadenosine-5'-phosphate (PAP) as a sulfate acceptor. The resultant Cypridina luciferin is used for the luciferase-luciferin reaction of Cypridina to emit blue light. the addition of CoA to the crude extracts significantly stimulates luminescence intensity. CoA can act as a sulfate acceptor from Cypridina luciferyl sulfate. In addition, the sulfate group of Cypridina luciferyl sulfate is also transferred to adenosine 5'-monophosphate (5'-AMP) and adenosine 3'-monophosphate (3'-AMP) by the sulfotransferase. Other adenosine derivatives of 5'-AMP and 3'-AMP can also act as efficient sulfate acceptors on the millimolar order from Cypridina luciferyl sulfate by the sulfotransferase and they stimulate light emission in crude extracts of Cypridina specimens. ATP, NADH, and NAD+ weakly stimulate the luminescence activity | Vargula hilgendorfii |