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Literature summary for 2.8.2.1 extracted from

  • Tibbs, Z.; Falany, C.
    An engineered heterodimeric model to investigate SULT1B1 dependence on intersubunit communication (2016), Biochem. Pharmacol., 115, 123-133 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.1

Protein Variants

Protein Variants Comment Organism
E275K generation of a targeted point mutation of the KxxxTVxxxE dimerization motif to KxxxTVxxxK (E275K) or ExxxTVxxxE (K266E). The heterodimer exhibits wild-type-like activity with regards to native size, thermal integrity, phosphoadenosine 5'-phosphate affinity, and phosphoadenosine 5'-phosphosulfate Km value. Substitution of the dysfunctional subunits for fully active subunits yields dimeric SULT1B1 with 50% the activity of the fully competent dimer Homo sapiens
H109Y mutation negatively affects 3'-phosphoadenosine 5'-phosphate binding by approximately 10fold and completely abolishes activity Homo sapiens
K266E generation of a targeted point mutation of the KxxxTVxxxE dimerization motif to KxxxTVxxxK (E275K) or ExxxTVxxxE (K266E). The heterodimer exhibits wild-type-like activity with regards to native size, thermal integrity, phosphoadenosine 5'-phosphate affinity, and phosphoadenosine 5'-phosphosulfate Km value. Substitution of the dysfunctional subunits for fully active subunits yields dimeric SULT1B1 with 50% the activity of the fully competent dimer Homo sapiens
R258K mutation renders the isoform unable to bind 3'-phosphoadenosine 5'-phosphate at detectible levels Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00158
-
 3'-phosphoadenylyl sulfate mutant E275K, pH 7.4, 37°C Homo sapiens
0.0019
-
 3'-phosphoadenylyl sulfate heterodimer of mutants K266E and E275K, pH 7.4, 37°C Homo sapiens
0.00197
-
 3'-phosphoadenylyl sulfate wild-type, pH 7.4, 37°C Homo sapiens
0.00388
-
 3'-phosphoadenylyl sulfate mutant K266E, pH 7.4, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 gel filtration, mutant K266E Homo sapiens
60000
-
 gel filtration, mutant E275K Homo sapiens
77000
-
 gel filtration, wild-type Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O43704 isoform SULT1B1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + 1-naphthol
-
 Homo sapiens adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 38000, His-tagged wild-type protein, calculated from sequence Homo sapiens

Synonyms

Synonyms Comment Organism
SULT1B1
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 wild-type, stable for at least 90 min. The K266E mutant loses almost all activity after a 90 minute 45°C incubation, the E275K mutant loses 30% of its sulfation activity. A mutants K266E/E275K heterodimer retains most of its activity after 45°C incubation for 90 min Homo sapiens