Literature summary for 2.8.2.1 extracted from

Itaeaho, K.; Alakurtti, S.; Yli-Kauhaluoma, J.; Taskinen, J.; Coughtrie, M.W.; Kostiainen, R.
Regioselective sulfonation of dopamine by SULT1A3 in vitro provides a molecular explanation for the preponderance of dopamine-3-O-sulfate in human blood circulation (2007), Biochem. Pharmacol., 74, 504-510.

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Cloned(Commentary)

Cloned (Comment)	Organism
SULT1A3, expression in Escherichia coli	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
dopamine 3-O-sulfate	35-85% product inhibition of SULT1A3	Homo sapiens
dopamine 4-O-sulfate	40-80% product inhibition of SULT1A3	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	SULT1A3, kinetics	Homo sapiens
0.0022	-	dopamine	dopamine 4-O-sulfate formation, recombinant SULT1A3	Homo sapiens
0.0026	-	dopamine	dopamine 3-O-sulfate formation, recombinant SULT1A3	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2 3'-phosphoadenylyl sulfate + 2 dopamine	Homo sapiens	SULT1A3	2 adenosine 3',5'-bisphosphate + dopamine 3-O-sulfate + dopamine 4-O-sulfate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P0DMM9	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant SULT1A3 from Escherichia coli strain by ammonium sulfate fractionation, anion exchange and adenosine 3',5'-bisphosphate affinity chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	development of a HPLC-based dopamine 3-O-sulfate and dopamine 4-O-sulfate product detection method, the formation of the dopamine 3-O-sulfate is much higher with SULT1A3 than the dopamine 4-O-sulfate formation, overview	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 3'-phosphoadenylyl sulfate + 2 dopamine	SULT1A3	Homo sapiens	2 adenosine 3',5'-bisphosphate + dopamine 3-O-sulfate + dopamine 4-O-sulfate	-	?
2 3'-phosphoadenylyl sulfate + 2 dopamine	SULT1A3	Homo sapiens	2 adenosine 3',5'-bisphosphate + dopamine 3-O-sulfate + dopamine 4-O-sulfate	NMR product structure analysis, dopamine 3-O-sulfate is the main product, overview	?

Synonyms

Synonyms	Comment	Organism
SULT1A3	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.8	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)