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Literature summary for 2.8.2.1 extracted from

  • Tsoi, C.; Widersten, M.; Morgenstern, R.; Swedmark, S.
    Amino acid residue 247 in canine sulphotransferase SULT1D1: a new determinant of substrate selectivity (2004), Biochem. J., 378, 687-692.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.1

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant enzymes Canis lupus familiaris

Protein Variants

Protein Variants Comment Organism
A146D sulfation activity of the mutant is strongly decreased for the substrates 4-nitrophenol and dopamine, enzyme form SULT1D1 Canis lupus familiaris
A146Q sulfation activity of the mutant is strongly decreased for the substrates 4-nitrophenol and dopamine, enzyme form SULT1D1 Canis lupus familiaris
D247L 21fold better sulfation of 4-nitrophenol (120fold decrease in KM-value) and 54fold less efficient in sulfation of dopamine (8fold increase in KM-value) compared to wild-type enzyme. Preference is switched from dopamine to 4-nitrophenol, enzyme form SULT1D1 Canis lupus familiaris
I86D sulfation activity of the mutant is strongly decreased for the substrates 4-nitrophenol and dopamine, enzyme form SULT1D1 Canis lupus familiaris

Inhibitors

Inhibitors Comment Organism Structure
4-nitrophenol substrate inhibition, enzyme form SULT1D1 Canis lupus familiaris
dopamine substrate inhibition, enzyme form SULT1D1 Canis lupus familiaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0015
-
 4-nitrophenol mutant enzyme D247L, enzyme form SULT1D1 Canis lupus familiaris
0.0036
-
 dopamine wild-type enzyme, enzyme form SULT1D1 Canis lupus familiaris
0.031
-
 dopamine mutant enzyme D247L, enzyme form SULT1D1 Canis lupus familiaris
0.037
-
 dopamine mutant enzyme O86D, enzyme form SULT1D1 Canis lupus familiaris
0.18
-
 4-nitrophenol wild-type enzyme, enzyme form SULT1D1 Canis lupus familiaris
1.6
-
 4-nitrophenol mutant enzyme O86D, enzyme form SULT1D1 Canis lupus familiaris

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
 enzyme form SULT1D1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Canis lupus familiaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + 4-nitrophenol enzyme form SULT1D1 Canis lupus familiaris adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?
3'-phosphoadenylylsulfate + dopamine enzyme form SULT1D1 Canis lupus familiaris adenosine 3',5'-bisphosphate + ?
-
 ?

Synonyms

Synonyms Comment Organism
SULT1D1
-
 Canis lupus familiaris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.026
-
 4-nitrophenol mutant enzyme D247L, enzyme form SULT1D1 Canis lupus familiaris
0.028
-
 dopamine mutant enzyme D247L, enzyme form SULT1D1 Canis lupus familiaris
0.089
-
 4-nitrophenol mutant enzyme O86D, enzyme form SULT1D1 Canis lupus familiaris
0.09
-
 dopamine mutant enzyme O86D, enzyme form SULT1D1 Canis lupus familiaris
0.15
-
 4-nitrophenol wild-type enzyme, enzyme form SULT1D1 Canis lupus familiaris
0.18
-
 dopamine wild-type enzyme, enzyme form SULT1D1 Canis lupus familiaris

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.061
-
 dopamine substrate inhibition, wild-type enzyme, enzyme form SULT1D1 Canis lupus familiaris
0.063
-
 4-nitrophenol substrate inhibition, mutant enzyme D247L, enzyme form SULT1D1 Canis lupus familiaris
1
-
 4-nitrophenol substrate inhibition, wild-type enzyme, enzyme form SULT1D1 Canis lupus familiaris