Literature summary for 2.8.2.1 extracted from

Liu, M.C.; Suiko, M.; Sakakibara, Y.
Mutational analysis of the substrate binding/catalytic domains of human M form and P form phenol sulfotransferases (2000), J. Biol. Chem., 275, 13460-13464.

Search for more literature for 2.8.2.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type P-PST and M-PST and diverse mutants of both in Escherichia coli XL1-Blue	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
A146E	P-PST mutant by site-directed mutagenesis, highly reduced activity with dopamine, highly increased activity wih 4-nitrophenol	Homo sapiens
A86D/I89E	P-PST mutant by site-directed mutagenesis, highly reduced activity with dopamine, highly increased activity wih 4-nitrophenol	Homo sapiens
A86D/I89E/A146E	P-PST mutant by site-directed mutagenesis, no activity	Homo sapiens
D86A	M-PST mutant by site-directed mutagenesis, reduced activity	Homo sapiens
D86A/E89I	M-PST mutant by site-directed mutagenesis, reduced activity	Homo sapiens
D86A/E89I/E146A	M-PST mutant by site-directed mutagenesis, highly reduced activity with dopamine, and highly increased activity with 4-nitrophenol	Homo sapiens
E146A	M-PST mutant by site-directed mutagenesis, highly reduced activity with dopamine, and highly increased activity with 4-nitrophenol	Homo sapiens
E89I	M-PST mutant by site-directed mutagenesis, reduced activity	Homo sapiens
H108A	P-PST mutant by site-directed mutagenesis, no activity	Homo sapiens
H108A	M-PST mutant by site-directed mutagenesis, no activity with 4-nitrophenol, highly reduced activity with dopamine	Homo sapiens
H143Y	M-PST mutant by site-directed mutagenesis, reduced activity with dopamine, unaltered activity wih 4-nitrophenol	Homo sapiens
I89E	P-PST mutant by site-directed mutagenesis, highly reduced activity with dopamine, highly increased activity wih 4-nitrophenol	Homo sapiens
I89E/A146E	P-PST mutant by site-directed mutagenesis, highly reduced activity with dopamine, no activity wih 4-nitrophenol	Homo sapiens
K48M	P-PST and M-PST mutants by site-directed mutagenesis, no activity	Homo sapiens
K48M/H108A	M-PST mutant by site-directed mutagenesis, no activity	Homo sapiens
N85K	M-PST mutant by site-directed mutagenesis, reduced activity	Homo sapiens
N85K/D86A	M-PST mutant by site-directed mutagenesis, reduced activity with dopamine, unaltered activity wih 4-nitrophenol	Homo sapiens
N85K/E89I	M-PST mutant by site-directed mutagenesis, reduced activity	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	2 forms: simple phenol sulfating P-PST, monoamine sulfating M-PST	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-phosphoadenylyl sulfate + a phenol	-	Homo sapiens	adenosine 3',5'-bisphosphate + an aryl sulfate	-	?

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)