Cloned (Comment) | Organism |
---|---|
overexpression of alpha- and beta-enzyme form in Escherichia coli, at high expression levels, the beta-enzyme form becomes the majority, while the alpha-enzyme form increases only slightly compared to low level expression | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
alpha- and beta-enzyme form | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate | the overall physiological reaction is a transfer of the sulfate group between 2-naphthol and 4-nitrophenol with the intermediate formation of 3'-phosphoadenylyl sulfate | Rattus norvegicus |
Renatured (Comment) | Organism |
---|---|
the alpha-enzyme form renatures in presence of adenosine 3',5'-bisphosphate, while the beta-enzyme form renature only in absence of adenosine 3',5'-bisphosphate | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + a phenol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + an aryl sulfate | - |
? | |
3'-phosphoadenylylsulfate + 2-naphthol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 2-naphthyl sulfate | - |
r | |
3'-phosphoadenylylsulfate + 4-nitrophenol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | alpha- and beta-enzyme forms assemble through different folding processes | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
3'-phosphoadenylyl sulfate | the overall physiological reaction is a transfer of the sulfate group between 2-naphthol and 4-nitrophenol with the intermediate formation of 3'-phosphoadenylyl sulfate from adenosine 3',5'-bisphosphate | Rattus norvegicus | |
adenosine 3',5'-bisphosphate | the overall physiological reaction is a transfer of the sulfate group between 2-naphthol and 4-nitrophenol with the intermediate formation of 3'-phosphoadenylyl sulfate from adenosine 3',5'-bisphosphate | Rattus norvegicus |