Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, transient expression of Dopa/tyrosine sulfotransferase in COS-7 cells and Escherichia coli XL1-Blue | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,3',5-triiodo-L-thyronine | substrate inhibition at 0.25 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
dopamine | pH 9.3, 37°C | Rattus norvegicus | |
0.76 | - |
L-3,4-dihydroxyphenylalanine | pH 9.3, 37°C | Rattus norvegicus | |
3.44 | - |
D-3,4-dihydroxyphenylalanine | pH 9.3, 37°C | Rattus norvegicus | |
30.9 | - |
4-nitrophenol | pH 9.3, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P52847 | - |
- |
Purification (Comment) | Organism |
---|---|
764fold, to homogeneity | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0022 | - |
purified enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + a phenol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + an aryl sulfate | - |
? | |
3'-phosphoadenylylsulfate + 3,3',5'-triiodo-L-thyronine | low activity | Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + 3,3',5-triiodo-D-thyronine | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + 3,3',5-triiodo-L-thyronine | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + 3,5-diiodo-L-thyronine | low activity | Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + 4-nitrophenol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate | - |
? | |
3'-phosphoadenylylsulfate + D-3,4-dihydroxyphenylalanine | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + DL-tyrosine | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + DL-tyrosyl sulfate | - |
? | |
3'-phosphoadenylylsulfate + dopamine | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + L-3,4-dihydroxyphenylalanine | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + L-tyrosine | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + L-tyrosyl O4-sulfate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SULT | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.3 | - |
- |
Rattus norvegicus |