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Literature summary for 2.8.2.1 extracted from

  • Romain, Y.; Demassieux, S.; Carriere, S.
    Partial purification and characterization of two isoenzymes involved in the sulfurylation of catecholamines (1982), Biochem. Biophys. Res. Commun., 106, 999-1005.
    View publication on PubMed
Search for more literature for 2.8.2.1

Activating Compound

Activating Compound Comment Organism Structure
EDTA 10 mM, stimulates isozymes A and B Canis lupus familiaris

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Canis lupus familiaris
Cu2+
-
 Canis lupus familiaris
KCl 50 mM, 60% inhibition of isoenzyme A, slight activation of isoenzyme B Canis lupus familiaris
Mg2+
-
 Canis lupus familiaris
Zn2+
-
 Canis lupus familiaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
 3'-phosphoadenylylsulfate with dopamine, isoenzyme A, pH 6.0, 37°C Canis lupus familiaris
0.0062
-
 dopamine isoenzyme B, pH 9.5, 37°C Canis lupus familiaris
0.0177
-
 dopamine isoenzyme A, pH 6.0, 37°C Canis lupus familiaris
0.026
-
 3'-phosphoadenylylsulfate with dopamine, isoenzyme B, pH 9.5, 37°C Canis lupus familiaris

Metals/Ions

Metals/Ions Comment Organism Structure
KCl
-
 Canis lupus familiaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 gel filtration Canis lupus familiaris

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
 isozymes A and B
-

Purification (Commentary)

Purification (Comment) Organism
isozyme A 18fold, isozyme B 35fold Canis lupus familiaris

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Canis lupus familiaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0016
-
 purified isozyme A Canis lupus familiaris
0.0036
-
 purified isozyme B Canis lupus familiaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + a phenol
-
 Canis lupus familiaris adenosine 3',5'-bisphosphate + an aryl sulfate
-
 ?
3'-phosphoadenylylsulfate + 2-hydroxyestradiol
-
 Canis lupus familiaris adenosine 3',5'-bisphosphate + estradiol 2-sulfate
-
 ?
3'-phosphoadenylylsulfate + 2-hydroxyestrone
-
 Canis lupus familiaris adenosine 3',5'-bisphosphate + estrone 2-sulfate
-
 ?
3'-phosphoadenylylsulfate + 4-nitrophenol
-
 Canis lupus familiaris adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?
3'-phosphoadenylylsulfate + adrenaline
-
 Canis lupus familiaris adenosine 3',5'-bisphosphate + adrenaline sulfate
-
 ?
3'-phosphoadenylylsulfate + dopamine
-
 Canis lupus familiaris adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + norepinephrine i.e. noradrenalin Canis lupus familiaris adenosine 3',5'-bisphosphate + norepinephrine sulfate i.e. noradrenalin sulfate ?
additional information isoenzyme B catalyzes the sulfurylation of a wider range of substrates than A which is preferentially active with dopamine Canis lupus familiaris ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Canis lupus familiaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 with substrate 4-nitrophenol, isoenzyme B Canis lupus familiaris
6
-
 with substrate dopamine, isoenzyme A Canis lupus familiaris
6.5
-
 isoenzyme A Canis lupus familiaris
6.5
-
 with substrate 4-nitrophenol Canis lupus familiaris
9.5
-
 with substrate dopamine, isoenzyme B Canis lupus familiaris

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
-
 Canis lupus familiaris