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Literature summary for 2.8.1.B3 extracted from

  • Neumann, P.; Lakomek, K.; Naumann, P.T.; Erwin, W.M.; Lauhon, C.T.; Ficner, R.
    Crystal structure of a 4-thiouridine synthetase-RNA complex reveals specificity of tRNA U8 modification (2014), Nucleic Acids Res., 42, 6673-6685.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.1.B3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with a truncated tRNA, sitting drop vapor diffusion method, using 2 M sodium formate, 100 mM sodium citrate, pH 4.6, 2 mM dithiothreitol Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8) Thermotoga maritima
-
 sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP
-
 ?
sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8) Thermotoga maritima ATCC 43589
-
 sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP
-
 ?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9X220
-
-
Thermotoga maritima ATCC 43589 Q9X220
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8)
-
 Thermotoga maritima sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP
-
 ?
sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8)
-
 Thermotoga maritima ATCC 43589 sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP
-
 ?

Synonyms

Synonyms Comment Organism
4-thiouridine synthetase
-
 Thermotoga maritima
ThiI
-
 Thermotoga maritima