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Literature summary for 2.8.1.B3 extracted from
- The role of the cysteine residues of ThiI in the generation of 4-thiouridine in tRNA (2001), J. Biol. Chem., 276, 33588-33595.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C108A | mutation has no significant effect on ThiI activity | Escherichia coli |
| C202A | mutation has no significant effect on ThiI activity | Escherichia coli |
| C344A | mutation results in the loss of function in vivo and a 2700fold reduction in activity measured in vitro | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8) | two mechanisms for the sulfur transfer mediated by ThiI are proposed: A. mechanism providing for the direct nucleophilic attack on an activated uridine species by the terminal sulfur of the persulfide on Cys-456 of ThiI. B. mechanism in which ThiI functions to generate hydrogen sulfide, which subsequently serves as the nucleophile to displace the activated oxygen of uridine 8 | Escherichia coli | sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ThiI | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Escherichia coli |
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Release 2023.1 (January 2023)
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