Any feedback?
Literature summary for 2.8.1.B3 extracted from
- Evidence for the transfer of sulfane sulfur from IscS to ThiI during the in vitro biosynthesis of 4-thiouridine in Escherichia coli tRNA (2000), J. Biol. Chem., 275, 10727-10730.
General Stability
| General Stability | Organism |
|---|---|
| tRNA protects ThiI from proteolysis | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-[((2-iodoacetamido)ethyl)amino]naphthalene sulfonic acid | irreversible inhibition. Both ATP and tRNA can protect ThiI from inhibition | Escherichia coli |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8) | Escherichia coli | ThiI is a recipient of S(0) from IscS and catalyzes the ultimate sulfur transfer step in the biosynthesis of 4-thiouridine | sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8) | ThiI is a recipient of S(0) from IscS and catalyzes the ultimate sulfur transfer step in the biosynthesis of 4-thiouridine | Escherichia coli | sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP | - |
? | |
| sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8) | sulfane sulfur generated by IscS is transferred sequentially to ThiI and then to tRNA during the in vitro synthesis of 4-thiouridine | Escherichia coli | sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ThiI | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
