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Literature summary for 2.8.1.B3 extracted from

  • Wright, C.M.; Christman, G.D.; Snellinger, A.M.; Johnston, M.V.; Mueller, E.G.
    Direct evidence for enzyme persulfide and disulfide intermediates during 4-thiouridine biosynthesis (2006), Chem. Commun. (Camb. ), 2006, 3104-3106.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C108/202/207/344A the mutant enzyme is 2900fold less active than the wild-type enzyme Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8) Cys456 bears a persulfide group upon incubation with sulfurtransferase IscS and cysteine, no intermediate sulfur carrier protein is chemically required Escherichia coli sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP
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