BRENDA - Enzyme Database show
show all sequences of 2.8.1.8

Crystallographic snapshots of sulfur insertion by lipoyl synthase

McLaughlin, M.I.; Lanz, N.D.; Goldman, P.J.; Lee, K.H.; Booker, S.J.; Drennan, C.L.; Proc. Natl. Acad. Sci. USA 113, 9446-9450 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Mycobacterium tuberculosis
Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion method, using 0.1 M HEPES, pH 7.5, 12% (w/v) PEG 10000, 7.5% (v/v) 2-methyl-2,4-pentanediol
Mycobacterium tuberculosis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycobacterium tuberculosis
P9WK91
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography and Sephacryl S-200 gel filtration
Mycobacterium tuberculosis
Cofactor
Cofactor
Commentary
Organism
Structure
4Fe-4S-center
-
Mycobacterium tuberculosis
S-adenosyl-L-methionine
-
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Mycobacterium tuberculosis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
4Fe-4S-center
-
Mycobacterium tuberculosis
S-adenosyl-L-methionine
-
Mycobacterium tuberculosis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, using 0.1 M HEPES, pH 7.5, 12% (w/v) PEG 10000, 7.5% (v/v) 2-methyl-2,4-pentanediol
Mycobacterium tuberculosis
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography and Sephacryl S-200 gel filtration
Mycobacterium tuberculosis
Other publictions for EC 2.8.1.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737720
Lanz
Characterization of Lipoyl Syn ...
Mycobacterium tuberculosis
Biochemistry
55
1372-1383
2016
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7
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739557
McLaughlin
Crystallographic snapshots of ...
Mycobacterium tuberculosis
Proc. Natl. Acad. Sci. USA
113
9446-9450
2016
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1
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726128
Ewald
Two redundant octanoyltransfer ...
Arabidopsis thaliana
Plant Biol.
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35-42
2014
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2
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737696
Lanz
Evidence for a catalytically a ...
Escherichia coli, Thermus thermophilus
Biochemistry
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4557-4572
2014
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8
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718133
Martin
A novel two-gene requirement f ...
Bacillus subtilis
Mol. Microbiol.
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1
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711217
Christensen
Lipoic acid synthesis: a new f ...
Bacillus subtilis
Biochemistry
49
10024-10036
2010
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Bryant
The activity of a thermostable ...
Saccharolobus solfataricus
Anal. Biochem.
351
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2006
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1
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671329
Douglas
Lipoyl synthase inserts sulfur ...
Saccharolobus solfataricus
Angew. Chem.
45
5197-5199
2006
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661945
Cicchillo
Mechanistic investigations of ...
Escherichia coli
J. Am. Chem. Soc.
127
2860-2861
2005
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661138
Cicchillo
Lipoyl synthase requires two e ...
Escherichia coli
Biochemistry
43
6378-6386
2004
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661463
Zhao
Assembly of the covalent linka ...
Escherichia coli
Chem. Biol.
10
1293-1302
2003
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661727
Thomsen-Zieger
Apicomplexan parasites contain ...
Toxoplasma gondii
FEBS Lett.
547
80-86
2003
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1
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663290
Kriek
Effect of iron-sulfur cluster ...
Escherichia coli
Protein Expr. Purif.
28
241-245
2003
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1
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661057
Miller
Escherichia coli LipA is a lip ...
Escherichia coli
Biochemistry
39
15166-15178
2000
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661718
Ollagnier-de Choudens
The lipoate synthase from Esch ...
Escherichia coli
FEBS Lett.
453
25-28
1999
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1
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662098
Jordan
A new metabolic link. The acyl ...
Escherichia coli, Neurospora crassa, Pisum sativum
J. Biol. Chem.
272
17903-17906
1997
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661982
vanden Boom
Lipoic acid metabolism in Esch ...
Escherichia coli
J. Bacteriol.
173
6411-6420
1991
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