BRENDA - Enzyme Database show
show all sequences of 2.8.1.8

Two redundant octanoyltransferases and one obligatory lipoyl synthase provide protein-lipoylation autonomy to plastids of Arabidopsis

Ewald, R.; Hoffmann, C.; Neuhaus, E.; Bauwe, H.; Plant Biol. 16, 35-42 (2014)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
plastid
-
Arabidopsis thaliana
9536
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
protein N6-(octanoyl)lysine + sulfur + S-adenosyl-L-methionine
Arabidopsis thaliana
-
protein N6-(lipoyl)lysine + L-methionine + 5'-deoxyadenosine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9ZWT1
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
protein N6-(octanoyl)lysine + sulfur + S-adenosyl-L-methionine
-
726128
Arabidopsis thaliana
protein N6-(lipoyl)lysine + L-methionine + 5'-deoxyadenosine
-
-
-
?
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
plastid
-
Arabidopsis thaliana
9536
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
protein N6-(octanoyl)lysine + sulfur + S-adenosyl-L-methionine
Arabidopsis thaliana
-
protein N6-(lipoyl)lysine + L-methionine + 5'-deoxyadenosine
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
protein N6-(octanoyl)lysine + sulfur + S-adenosyl-L-methionine
-
726128
Arabidopsis thaliana
protein N6-(lipoyl)lysine + L-methionine + 5'-deoxyadenosine
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
the knockout of isoform LIP1p is fatal for early embryo development
Arabidopsis thaliana
physiological function
isoform LIP1 is indispensable for embryo development and essential for plastids
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
malfunction
the knockout of isoform LIP1p is fatal for early embryo development
Arabidopsis thaliana
physiological function
isoform LIP1 is indispensable for embryo development and essential for plastids
Arabidopsis thaliana
Other publictions for EC 2.8.1.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737720
Lanz
Characterization of Lipoyl Syn ...
Mycobacterium tuberculosis
Biochemistry
55
1372-1383
2016
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739557
McLaughlin
Crystallographic snapshots of ...
Mycobacterium tuberculosis
Proc. Natl. Acad. Sci. USA
113
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2016
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726128
Ewald
Two redundant octanoyltransfer ...
Arabidopsis thaliana
Plant Biol.
16
35-42
2014
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737696
Lanz
Evidence for a catalytically a ...
Escherichia coli, Thermus thermophilus
Biochemistry
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4557-4572
2014
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Martin
A novel two-gene requirement f ...
Bacillus subtilis
Mol. Microbiol.
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2011
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711217
Christensen
Lipoic acid synthesis: a new f ...
Bacillus subtilis
Biochemistry
49
10024-10036
2010
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660704
Bryant
The activity of a thermostable ...
Saccharolobus solfataricus
Anal. Biochem.
351
44-49
2006
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671329
Douglas
Lipoyl synthase inserts sulfur ...
Saccharolobus solfataricus
Angew. Chem.
45
5197-5199
2006
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661945
Cicchillo
Mechanistic investigations of ...
Escherichia coli
J. Am. Chem. Soc.
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2860-2861
2005
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661138
Cicchillo
Lipoyl synthase requires two e ...
Escherichia coli
Biochemistry
43
6378-6386
2004
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661463
Zhao
Assembly of the covalent linka ...
Escherichia coli
Chem. Biol.
10
1293-1302
2003
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661727
Thomsen-Zieger
Apicomplexan parasites contain ...
Toxoplasma gondii
FEBS Lett.
547
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2003
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663290
Kriek
Effect of iron-sulfur cluster ...
Escherichia coli
Protein Expr. Purif.
28
241-245
2003
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661057
Miller
Escherichia coli LipA is a lip ...
Escherichia coli
Biochemistry
39
15166-15178
2000
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661718
Ollagnier-de Choudens
The lipoate synthase from Esch ...
Escherichia coli
FEBS Lett.
453
25-28
1999
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662098
Jordan
A new metabolic link. The acyl ...
Escherichia coli, Neurospora crassa, Pisum sativum
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17903-17906
1997
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661982
vanden Boom
Lipoic acid metabolism in Esch ...
Escherichia coli
J. Bacteriol.
173
6411-6420
1991
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