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Literature summary for 2.8.1.7 extracted from
- Molecular basis of function and the unusual antioxidant activity of a cyanobacterial cysteine desulfurase (2017), Biochem. J., 474, 2435-2447 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Anabaena sulfur acceptor E | in the presence of the Anabaena sulfur acceptor E protein, the catalytic efficiency increases 10fold. For sulfur mobilization, the Anabaena sulfur acceptor E protein partners only SufS and not other cysteine desulfurases from Nostoc sp. | Nostoc sp. PCC 7120 = FACHB-418 |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| homology modeling of structure | Nostoc sp. PCC 7120 = FACHB-418 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C374S | complete loss of sulfur desulfurase activity | Nostoc sp. PCC 7120 = FACHB-418 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.152 | - |
L-cysteine | absence of Anabaena sulfur acceptor E, pH 7.4, temperature not specified in the publication | Nostoc sp. PCC 7120 = FACHB-418 |  |
| 0.187 | - |
L-cysteine | presence of Anabaena sulfur acceptor E, pH 7.4, temperature not specified in the publication | Nostoc sp. PCC 7120 = FACHB-418 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nostoc sp. PCC 7120 = FACHB-418 | Q8YU59 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine | - |
Nostoc sp. PCC 7120 = FACHB-418 | L-alanine + H2S | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alr2495 | - |
Nostoc sp. PCC 7120 = FACHB-418 |
| SufS | - |
Nostoc sp. PCC 7120 = FACHB-418 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.136 | - |
L-cysteine | absence of Anabaena sulfur acceptor E, pH 7.4, temperature not specified in the publication | Nostoc sp. PCC 7120 = FACHB-418 | |
| 1.427 | - |
L-cysteine | presence of Anabaena sulfur acceptor E, pH 7.4, temperature not specified in the publication | Nostoc sp. PCC 7120 = FACHB-418 | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Nostoc sp. PCC 7120 = FACHB-418 | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | SufS protein physically interacts with the Anabaena sulfur acceptor E protein. In the presence of the Anabaena sulfur acceptor E protein, the catalytic efficiency increases 10fold. For sulfur mobilization, the Anabaena sulfur acceptor E protein partners only SufS and not other cysteine desulfurases from Nostoc sp. The conserved cysteine of the SufS or the Anabaena sulfur acceptor E protein is essential for activity but not for their physical association | Nostoc sp. PCC 7120 = FACHB-418 |
| physiological function | overexpression of the SufS protein causes reduced formation of reactive oxygen species on exposure to hydrogen peroxide | Nostoc sp. PCC 7120 = FACHB-418 |
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