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Literature summary for 2.8.1.7 extracted from

  • Liu, Y.; Lei, X.Y.; Chen, L.F.; Bian, Y.B.; Yang, H.; Ibrahim, S.A.; Huang, W.
    A novel cysteine desulfurase influencing organosulfur compounds in Lentinula edodes (2015), Sci. Rep., 5, 10047.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Lentinula edodes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.72
-
 L-cysteine at pH 8.4 and 37°C Lentinula edodes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 x * 54000, SDS-PAGE Lentinula edodes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-cysteine + acceptor Lentinula edodes
-
 L-alanine + S-sulfanyl-acceptor
-
 ?
additional information Lentinula edodes the enzyme Lecsl has both cysteine sulfoxide lyase and cysteine desulfurase activity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lentinula edodes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel Hi-trap column chromatography Lentinula edodes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + acceptor
-
 Lentinula edodes L-alanine + S-sulfanyl-acceptor
-
 ?
additional information the enzyme Lecsl has both cysteine sulfoxide lyase and cysteine desulfurase activity Lentinula edodes ?
-
 ?

Subunits

Subunits Comment Organism
? x * 54000, SDS-PAGE Lentinula edodes

Synonyms

Synonyms Comment Organism
Lecsl
-
 Lentinula edodes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.01
-
 L-cysteine at pH 8.4 and 37°C Lentinula edodes

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Lentinula edodes

pI Value

Organism Comment pI Value Maximum pI Value
Lentinula edodes isoelectric focusing
-
 6.1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.586
-
 L-cysteine at pH 8.4 and 37°C Lentinula edodes