Literature summary for 2.8.1.7 extracted from

Chen, F.; Zhang, Z.; Lin, K.; Qian, T.; Zhang, Y.; You, D.; He, X.; Wang, Z.; Liang, J.; Deng, Z.; Wu, G.
Crystal structure of the cysteine desulfurase DndA from Streptomyces lividans which is involved in DNA phosphorothioation (2012), PLoS ONE, 7, e36635.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Streptomyces lividans

Crystallization (Commentary)

Crystallization (Comment)	Organism
C327S mutant in complex with pyridoxal 5'-phosphate and in the absence of L-cysteine, hanging drop vapor diffusion method, using 20% (w/v) PEG 3350, 0.1 M sodium acetate, pH 5.5, and 0.2 M ammonium citrate	Streptomyces lividans

Protein Variants

Protein Variants	Comment	Organism
C327S	inactive	Streptomyces lividans
C327S/S329C	inactive	Streptomyces lividans
S325C/C327S	inactive	Streptomyces lividans

Organism

Organism	UniProt	Comment	Textmining
Streptomyces lividans	A7TUX7	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-NTA affinity column chromatography, Source 30Q anion exchange chromatography, and Superdex 200 gel filtration	Streptomyces lividans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-cysteine + [enzyme]-cysteine	-	Streptomyces lividans	L-alanine + [enzyme]-S-sulfanylcysteine	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 000, calculated from amino acid sequence	Streptomyces lividans

Synonyms

Synonyms	Comment	Organism
DndA	-	Streptomyces lividans

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Streptomyces lividans

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Release 2023.1 (January 2023)