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Literature summary for 2.8.1.7 extracted from
- A novel DNA modification by sulfur: DndA is a NifS-like cysteine desulfurase capable of assembling DndC as an iron-sulfur cluster protein in Streptomyces lividans (2007), Biochemistry, 46, 6126-6133.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Streptomyces lividans |
| expression in Escherichia coli BL21(DE3) pLysE, expression vector pET15b | Streptomyces lividans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C327S | no residual cysteine desulfurase activity, mutant is not able to reactivate apo-Fe DndC protein | Streptomyces lividans |
| C327S | sited-directed mutagenesis, no cysteine desulfurase activity, no ability to reactivate the apo-Fe DndC | Streptomyces lividans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.36 | - |
L-selenocysteine | pH 8.0, 37°C | Streptomyces lividans |  |
| 5.1 | - |
L-cysteine | pH 8.0, 37°C | Streptomyces lividans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | a single [4Fe-4S] cluster per DndC monomer | Streptomyces lividans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
subunit, SDS-PAGE | Streptomyces lividans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine | Streptomyces lividans | DndA is able to directly activate apo-Fe DndC for its reconstitution as a fully functional [4Fe-4S] cluster protein (DndC) with unambiguously demonstrated ATP pyrophosphatase activity | L-alanine + sulfur | - |
? | |
| additional information | Streptomyces lividans | substrate specificity similar to that of Escherichia coli IscS | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces lividans | - |
recombinant protein | - |
| Streptomyces lividans | Q460H9 | expression in Escherichia coli BL21(DE3) pLysE, expression vector pET15b | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity with a HiTrap chelating column | Streptomyces lividans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
no detectable activity for L-selenocystine and L-cystine | Streptomyces lividans |
| 3.6 | - |
substrate L-cysteine sulfinic acid, pH 8.0, 37°C | Streptomyces lividans |
| 38.6 | - |
substrate L-cysteine, pH 8.0, 37°C | Streptomyces lividans |
| 38.6 | - |
substrate L-selenocysteine, pH 8.0, 37°C | Streptomyces lividans |
| 56.5 | - |
substrate L-cysteine, pH 8.0, 37°C | Streptomyces lividans |
| 56.5 | - |
substrate L-selenocysteine, pH 8.0, 37°C | Streptomyces lividans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine | DndA is able to directly activate apo-Fe DndC for its reconstitution as a fully functional [4Fe-4S] cluster protein (DndC) with unambiguously demonstrated ATP pyrophosphatase activity | Streptomyces lividans | L-alanine + sulfur | - |
? | |
| L-cysteine + DndA | - |
Streptomyces lividans | L-alanine + DndA-SSH | - |
? | |
| L-cysteine sulfinic acid + ? | cysteine desulfurase DndA catalyzes iron-sulfur cluster assembly by activation of apo-Fe DndC protein | Streptomyces lividans | ? | - |
? | |
| L-cysteine sulfinic acid + DndA | - |
Streptomyces lividans | ? | - |
? | |
| L-selenocysteine + ? | - |
Streptomyces lividans | ? | - |
? | |
| L-selenocysteine + DndA | - |
Streptomyces lividans | L-alanine + DndA-SSeH | - |
? | |
| additional information | cysteine desulfurase DndA catalyzes iron-sulfur cluster assembly by activation of apo-Fe DndC protein | Streptomyces lividans | ? | - |
? | |
| additional information | no substrate: L-cystine, L-selenocystine | Streptomyces lividans | ? | - |
? | |
| additional information | substrate specificity similar to that of Escherichia coli IscS | Streptomyces lividans | ? | - |
? | |
| additional information | no substrates are L-cystine, L-selenocystine | Streptomyces lividans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | subunit 45000, homodimer 91000, purified soluble DndA protein, gel filtration with a Superose 12 10/30 column | Streptomyces lividans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cysteine desulfurase | - |
Streptomyces lividans |
| DndA | - |
Streptomyces lividans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
L-cysteine | pH 8.0, 37°C | Streptomyces lividans | |
| 1.4 | - |
L-selenocysteine | pH 8.0, 37°C | Streptomyces lividans | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Streptomyces lividans | |
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