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Literature summary for 2.8.1.6 extracted from

  • Magwamba, C.C.; Rukseree, K.; Palittapongarnpim, P.
    Cloning, expression and characterization of histidine-tagged biotin synthase of Mycobacterium tuberculosis (2016), Tuberculosis, 98, 42-49 .
    View publication on PubMed
Search for more literature for 2.8.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
5'-deoxyadenosine competitive, inhibits the growth of Mycobacterium tuberculosis H37Ra with an MIC of 392.6 microg/ml Mycobacterium tuberculosis
S-(5'-adenosyl)-L-cysteine competitive Mycobacterium tuberculosis
S-(5'-adenosyl)-L-homocysteine competitive Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00281
-
 dethiobiotin pH 8.0, 37°C Mycobacterium tuberculosis
0.00995
-
 S-adenosyl-L-methionine pH 8.0, 37°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WPQ7
-
-
Mycobacterium tuberculosis H37Rv P9WPQ7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin
-
 Mycobacterium tuberculosis biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin
-
 ?
dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin
-
 Mycobacterium tuberculosis H37Rv biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin
-
 ?

Subunits

Subunits Comment Organism
? x* 38500, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
BioB
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0016
-
 dethiobiotin pH 8.0, 37°C Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000592
-
 S-(5'-adenosyl)-L-homocysteine pH 8.0, 37°C Mycobacterium tuberculosis
0.00084
-
 S-(5'-adenosyl)-L-cysteine pH 8.0, 37°C Mycobacterium tuberculosis
0.0242
-
 5'-deoxyadenosine pH 8.0, 37°C Mycobacterium tuberculosis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00645
-
 pH 8.0, 37°C Mycobacterium tuberculosis S-(5'-adenosyl)-L-homocysteine
0.00928
-
 pH 8.0, 37°C Mycobacterium tuberculosis S-(5'-adenosyl)-L-cysteine
0.2674
-
 pH 8.0, 37°C Mycobacterium tuberculosis 5'-deoxyadenosine