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Literature summary for 2.8.1.6 extracted from
- Biotin synthase of Bacillus subtilis shows less reactivity than that of Escherichia coli in in vitro reaction systems (2002), Arch. Microbiol., 179, 26-32.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | required | Escherichia coli |  |
| S-adenosyl-L-methionine | required | Bacillus subtilis | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
- |
Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | enhances activity | Bacillus subtilis | |
| Fe2+ | enhances activity | Escherichia coli | |
| Iron | the enzyme contains an iron-sulfur cluster | Bacillus subtilis | |
| Iron | the enzyme contains an iron-sulfur cluster | Escherichia coli | |
| S2- | enhances activity | Bacillus subtilis | |
| S2- | enhances activity | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
2 * 37000, SDS-PAGE | Bacillus subtilis |
| 80000 | - |
gel filtration | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dethiobiotin + sulfur | - |
Bacillus subtilis | biotin | - |
? | |
| dethiobiotin + sulfur | - |
Escherichia coli | biotin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Escherichia coli |
| dimer | 2 * 37000, SDS-PAGE | Bacillus subtilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000005 | - |
dethiobiotin | pH 7.5, 30°C, cell free extract | Bacillus subtilis | |
| 0.0000142 | - |
dethiobiotin | pH 7.5, 30°C, cell free extract | Escherichia coli | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Escherichia coli | |
| NADPH | required | Bacillus subtilis | |
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