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Literature summary for 2.8.1.5 extracted from
- Identification and characterization of a multi-domain sulfurtransferase in Phanerochaete chrysosporium (2014), Biotechnol. Lett., 36, 993-999 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Phanerodontia chrysosporium |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C137A | complete loss of both sulfurtransferase and ATPase activity | Phanerodontia chrysosporium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
dithiothreitol | pH 8.0, 30°C | Phanerodontia chrysosporium |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phanerodontia chrysosporium | I3RJK8 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme additionally hydrolyzes ATP | Phanerodontia chrysosporium | ? | - |
? | |
| thiosulfate + 2 dithiothreitol | - |
Phanerodontia chrysosporium | sulfite + dithiothreitol disulfide + sulfide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 20680, calculated, x * 28000, SDS-PAGE | Phanerodontia chrysosporium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Sft | - |
Phanerodontia chrysosporium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Phanerodontia chrysosporium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
50 min, 68% residual activity | Phanerodontia chrysosporium |
| 40 | - |
50 min, 51% residual activity | Phanerodontia chrysosporium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5 | - |
dithiothreitol | pH 8.0, 30°C | Phanerodontia chrysosporium | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Phanerodontia chrysosporium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
50 min, 68% residual activity | Phanerodontia chrysosporium |
| 8.5 | - |
50 min, 56% residual activity | Phanerodontia chrysosporium |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Phanerodontia chrysosporium | calculated | - |
9.2 |
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