BRENDA - Enzyme Database
show all sequences of 2.8.1.5

Thiosulfate reductase from a moderately thermophilic iron-oxidizing bacterium, strain TI-1: purification and characterization

Sugio, T.; Kishimoto, K.; Oda, K.; Biosci. Biotechnol. Biochem. 61, 470-474 (1997)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
EDTA
-
thermophilic iron-oxidizing bacterium strain TI-1
o-phenanthroline
-
thermophilic iron-oxidizing bacterium strain TI-1
Sodium cyanide
2.5fold increase at 1 mM
thermophilic iron-oxidizing bacterium strain TI-1
Inhibitors
Inhibitors
Commentary
Organism
Structure
4-chloromercuribenzene sufonic acid
strong inhibition at 0.1 mM
thermophilic iron-oxidizing bacterium strain TI-1
Co2+
slight inhibition at 1 mM
thermophilic iron-oxidizing bacterium strain TI-1
Ni2+
slight inhibition at 1 mM
thermophilic iron-oxidizing bacterium strain TI-1
Zn2+
slight inhibition at 1 mM
thermophilic iron-oxidizing bacterium strain TI-1
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.125
-
NADH
pH 6, 45°C
thermophilic iron-oxidizing bacterium strain TI-1
0.29
-
thiosulfate
pH 6, 45°C
thermophilic iron-oxidizing bacterium strain TI-1
5
-
NADPH
pH 6, 45°C
thermophilic iron-oxidizing bacterium strain TI-1
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
thermophilic iron-oxidizing bacterium strain TI-1
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
homotetramer, 4 * 58000, SDS-PAGE
thermophilic iron-oxidizing bacterium strain TI-1
243000
-
gel filtration
thermophilic iron-oxidizing bacterium strain TI-1
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
thermophilic iron-oxidizing bacterium strain TI-1
-
-
-
Purification (Commentary)
Commentary
Organism
-
thermophilic iron-oxidizing bacterium strain TI-1
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
thiosulfate + NAD(P)H
-
645579
thermophilic iron-oxidizing bacterium strain TI-1
H2S + NAD(P)+ + sulfite
-
645579
thermophilic iron-oxidizing bacterium strain TI-1
?
Subunits
Subunits
Commentary
Organism
tetramer
homotetramer, 4 * 58000, SDS-PAGE
thermophilic iron-oxidizing bacterium strain TI-1
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
thermophilic iron-oxidizing bacterium strain TI-1
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
100
-
no enzyme activity after 15 min
thermophilic iron-oxidizing bacterium strain TI-1
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
thermophilic iron-oxidizing bacterium strain TI-1
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
EDTA
-
thermophilic iron-oxidizing bacterium strain TI-1
o-phenanthroline
-
thermophilic iron-oxidizing bacterium strain TI-1
Sodium cyanide
2.5fold increase at 1 mM
thermophilic iron-oxidizing bacterium strain TI-1
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
4-chloromercuribenzene sufonic acid
strong inhibition at 0.1 mM
thermophilic iron-oxidizing bacterium strain TI-1
Co2+
slight inhibition at 1 mM
thermophilic iron-oxidizing bacterium strain TI-1
Ni2+
slight inhibition at 1 mM
thermophilic iron-oxidizing bacterium strain TI-1
Zn2+
slight inhibition at 1 mM
thermophilic iron-oxidizing bacterium strain TI-1
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.125
-
NADH
pH 6, 45°C
thermophilic iron-oxidizing bacterium strain TI-1
0.29
-
thiosulfate
pH 6, 45°C
thermophilic iron-oxidizing bacterium strain TI-1
5
-
NADPH
pH 6, 45°C
thermophilic iron-oxidizing bacterium strain TI-1
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
thermophilic iron-oxidizing bacterium strain TI-1
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
homotetramer, 4 * 58000, SDS-PAGE
thermophilic iron-oxidizing bacterium strain TI-1
243000
-
gel filtration
thermophilic iron-oxidizing bacterium strain TI-1
Purification (Commentary) (protein specific)
Commentary
Organism
-
thermophilic iron-oxidizing bacterium strain TI-1
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
thiosulfate + NAD(P)H
-
645579
thermophilic iron-oxidizing bacterium strain TI-1
H2S + NAD(P)+ + sulfite
-
645579
thermophilic iron-oxidizing bacterium strain TI-1
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
homotetramer, 4 * 58000, SDS-PAGE
thermophilic iron-oxidizing bacterium strain TI-1
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
thermophilic iron-oxidizing bacterium strain TI-1
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
100
-
no enzyme activity after 15 min
thermophilic iron-oxidizing bacterium strain TI-1
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
thermophilic iron-oxidizing bacterium strain TI-1
Other publictions for EC 2.8.1.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Wang
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1
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1
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725273
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Thiosulfate reduction in Salmo ...
Salmonella enterica
J. Bacteriol.
194
475-485
2012
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1
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3
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1
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4
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3
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645578
Bang
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Cadmium, lead, and zinc remova ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biotechnol. Lett.
22
1331-1335
2000
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645577
Bagchi
-
Partial purification and regul ...
Phormidium uncinatum
Indian J. Exp. Biol.
35
876-880
1997
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1
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2
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1
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645579
Sugio
-
Thiosulfate reductase from a m ...
thermophilic iron-oxidizing bacterium strain TI-1
Biosci. Biotechnol. Biochem.
61
470-474
1997
3
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-
4
3
1
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2
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1
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1
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1
1
1
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1
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1
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3
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4
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3
1
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2
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1
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1
1
1
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1
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1
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645576
Akagi
-
Thiosulfate and trithionate re ...
Desulfotomaculum nigrificans, Desulfovibrio vulgaris
Methods Enzymol.
243
260-270
1994
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2
5
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1
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1
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1
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645575
Schmidt
Isolation and characterization ...
Chlorella fusca 211-8b, Chlorella fusca
Planta
162
243-249
1984
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1
5
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