BRENDA - Enzyme Database show
show all sequences of 2.8.1.5

Thiosulfate and trithionate reductases

Akagi, J.M.; Drake, H.L.; Kim, J.H.; Gevertz, D.; Methods Enzymol. 243, 260-270 (1994)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
enzyme is more stable in phosphate buffer than in Tris, borate or triethanolamine buffer
Desulfovibrio vulgaris
enzyme is more stable in phosphate buffer than in Tris, borate or triethanolamine buffer
Desulfotomaculum nigrificans
Inhibitors
Inhibitors
Commentary
Organism
Structure
2,2'-bipyridine
-
Desulfovibrio vulgaris
o-phenanthroline
-
Desulfovibrio vulgaris
Sulfhydryl agents
-
Desulfovibrio vulgaris
sulfite
-
Desulfotomaculum nigrificans
trithionate
-
Desulfovibrio vulgaris
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
16300
-
sedimentation equilibrium experiments
Desulfovibrio vulgaris
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Desulfotomaculum nigrificans
-
-
-
Desulfovibrio vulgaris
-
-
-
Oxidation Stability
Oxidation Stability
Organism
sensitive to oxygen
Desulfovibrio vulgaris
Purification (Commentary)
Commentary
Organism
-
Desulfovibrio vulgaris
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
thiosulfate + methylviologen
-
645576
Desulfovibrio vulgaris
sulfide + ?
-
-
-
?
thiosulfate + methylviologen
-
645576
Desulfotomaculum nigrificans
sulfide + ?
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Desulfovibrio vulgaris
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
enzyme contains FAD as prosthetic group
Desulfotomaculum nigrificans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
enzyme contains FAD as prosthetic group
Desulfotomaculum nigrificans
General Stability (protein specific)
General Stability
Organism
enzyme is more stable in phosphate buffer than in Tris, borate or triethanolamine buffer
Desulfovibrio vulgaris
enzyme is more stable in phosphate buffer than in Tris, borate or triethanolamine buffer
Desulfotomaculum nigrificans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,2'-bipyridine
-
Desulfovibrio vulgaris
o-phenanthroline
-
Desulfovibrio vulgaris
Sulfhydryl agents
-
Desulfovibrio vulgaris
sulfite
-
Desulfotomaculum nigrificans
trithionate
-
Desulfovibrio vulgaris
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
16300
-
sedimentation equilibrium experiments
Desulfovibrio vulgaris
Oxidation Stability (protein specific)
Oxidation Stability
Organism
sensitive to oxygen
Desulfovibrio vulgaris
Purification (Commentary) (protein specific)
Commentary
Organism
-
Desulfovibrio vulgaris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
thiosulfate + methylviologen
-
645576
Desulfovibrio vulgaris
sulfide + ?
-
-
-
?
thiosulfate + methylviologen
-
645576
Desulfotomaculum nigrificans
sulfide + ?
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Desulfovibrio vulgaris
Other publictions for EC 2.8.1.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742170
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Phanerochaete chrysosporium
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-
1
-
1
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-
1
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-
-
-
1
-
-
-
-
-
-
-
-
2
1
1
-
2
1
1
-
2
-
-
1
-
-
-
1
-
-
1
-
-
-
-
1
-
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-
-
-
-
-
-
-
-
-
2
1
1
-
2
1
1
-
2
1
-
-
-
-
-
-
725273
Stoffels
Thiosulfate reduction in Salmo ...
Salmonella enterica
J. Bacteriol.
194
475-485
2012
-
-
1
-
-
-
3
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
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-
-
-
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1
-
-
-
-
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3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645578
Bang
-
Cadmium, lead, and zinc remova ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biotechnol. Lett.
22
1331-1335
2000
-
-
1
-
-
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-
-
-
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1
-
1
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-
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2
-
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1
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-
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-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645577
Bagchi
-
Partial purification and regul ...
Phormidium uncinatum
Indian J. Exp. Biol.
35
876-880
1997
-
-
-
-
-
-
-
1
-
-
-
2
-
1
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645579
Sugio
-
Thiosulfate reductase from a m ...
thermophilic iron-oxidizing bacterium strain TI-1
Biosci. Biotechnol. Biochem.
61
470-474
1997
3
-
-
-
-
-
4
3
1
-
2
-
-
1
-
-
1
-
-
-
-
-
1
1
1
-
1
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
4
-
3
1
-
2
-
-
-
-
1
-
-
-
-
1
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
645576
Akagi
-
Thiosulfate and trithionate re ...
Desulfotomaculum nigrificans, Desulfovibrio vulgaris
Methods Enzymol.
243
260-270
1994
-
-
-
-
-
2
5
-
-
-
1
-
-
2
1
-
1
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
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1
-
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2
-
5
-
-
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-
1
-
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1
-
1
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-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
645575
Schmidt
Isolation and characterization ...
Chlorella fusca 211-8b, Chlorella fusca
Planta
162
243-249
1984
-
-
-
-
-
-
1
5
-
-
4
2
-
2
-
-
1
-
-
-
1
-
8
-
1
-
-
-
3
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
5
-
-
4
2
-
-
-
1
-
-
1
-
8
-
1
-
-
-
3
-
-
-
-
-
-
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-