BRENDA - Enzyme Database
show all sequences of 2.8.1.3

The catalytic mechanism of yeast thiosulfate reductase

Chauncey, T.R.; Westley, J.; J. Biol. Chem. 258, 15037-15045 (1983)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
benzene sulfinate
competitive with both substrates
Saccharomyces cerevisiae
sulfate
non-competitive inhibition when glutathione is the varied substrate
Saccharomyces cerevisiae
sulfite
competitive inhibition when glutathione is the varied substrate
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.24
-
Benzenethiosulfonate
pH 8.1, 37°C
Saccharomyces cerevisiae
0.89
-
glutathione
pH 8.1, 37°C, with benzenethiosulfonate as the other substrate
Saccharomyces cerevisiae
2.9
-
thiosulfate
pH 8.1, 37°C
Saccharomyces cerevisiae
4
-
glutathione
pH 8.1, 37°C, with thiosulfate as the other substrate
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
thiosulfate + glutathione
Saccharomyces cerevisiae
-
sulfite + glutathione disulfide + sulfide
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
Red Star brand
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzenethiosulfonate + glutathione
rapid equilibrium-ordered mechanism with glutathione as leading substrate
645572
Saccharomyces cerevisiae
additional information
glutathione persulfide as an immediate product
645572
Saccharomyces cerevisiae
?
thiosulfate + glutathione
-
645572
Saccharomyces cerevisiae
sulfite + glutathione disulfide + sulfide
-
-
-
?
thiosulfate + glutathione
-
645572
Saccharomyces cerevisiae
sulfite + glutathione disulfide + sulfide
the primary product is glutathione hydrodisulfide, which reacts with glutathione to give oxidized glutathione and sulfide
645572
Saccharomyces cerevisiae
?
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
benzene sulfinate
competitive with both substrates
Saccharomyces cerevisiae
sulfate
non-competitive inhibition when glutathione is the varied substrate
Saccharomyces cerevisiae
sulfite
competitive inhibition when glutathione is the varied substrate
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.24
-
Benzenethiosulfonate
pH 8.1, 37°C
Saccharomyces cerevisiae
0.89
-
glutathione
pH 8.1, 37°C, with benzenethiosulfonate as the other substrate
Saccharomyces cerevisiae
2.9
-
thiosulfate
pH 8.1, 37°C
Saccharomyces cerevisiae
4
-
glutathione
pH 8.1, 37°C, with thiosulfate as the other substrate
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
thiosulfate + glutathione
Saccharomyces cerevisiae
-
sulfite + glutathione disulfide + sulfide
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzenethiosulfonate + glutathione
rapid equilibrium-ordered mechanism with glutathione as leading substrate
645572
Saccharomyces cerevisiae
additional information
glutathione persulfide as an immediate product
645572
Saccharomyces cerevisiae
?
thiosulfate + glutathione
-
645572
Saccharomyces cerevisiae
sulfite + glutathione disulfide + sulfide
-
-
-
?
thiosulfate + glutathione
-
645572
Saccharomyces cerevisiae
sulfite + glutathione disulfide + sulfide
the primary product is glutathione hydrodisulfide, which reacts with glutathione to give oxidized glutathione and sulfide
645572
Saccharomyces cerevisiae
?
Other publictions for EC 2.8.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
645574
Aird
A physical characterization of ...
Acinetobacter calcoaceticus
Biochim. Biophys. Acta
1038
10-17
1990
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-
2
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-
1
-
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-
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-
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-
-
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-
-
-
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-
-
-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645519
Aird
Isolation and characterization ...
Acinetobacter calcoaceticus
J. Biol. Chem.
262
17327-17335
1987
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-
-
-
-
-
2
2
-
-
-
-
-
3
-
-
1
1
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-
1
-
2
-
-
-
-
-
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-
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-
1
-
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-
-
-
-
-
-
-
2
1
2
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645571
Chauncey
Improved purification and sulf ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
744
304-311
1983
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-
-
-
-
1
1
-
-
-
-
1
-
1
-
-
1
1
-
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
-
1
-
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645572
Chauncey
The catalytic mechanism of yea ...
Saccharomyces cerevisiae
J. Biol. Chem.
258
15037-15045
1983
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-
-
-
-
-
3
4
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
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-
-
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-
-
-
-
-
-
-
3
-
4
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-
1
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-
-
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-
-
-
3
-
-
-
-
-
-
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-
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-
645570
Uhteg
Purification and steady-state ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
195
211-222
1979
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-
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1
5
-
-
-
1
1
-
1
-
-
1
1
-
-
1
1
4
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
1
-
5
-
-
-
-
1
1
-
-
-
1
-
-
1
1
4
1
-
-
-
-
1
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-
1
-
-
-
-
-
-