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Literature summary for 2.8.1.2 extracted from

  • Fraesdorf, B.; Radon, C.; Leimkuehler, S.
    Characterization and interaction studies of two isoforms of the dual localized 3-mercaptopyruvate sulfurtransferase TUM1 from humans (2014), J. Biol. Chem., 289, 34543-34556.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.54
-
3-Mercaptopyruvate isoform TUM1-Iso2, with dithiothreitol as cosubstrate, at pH 10.5 and 37°C Homo sapiens
0.55
-
3-Mercaptopyruvate isoform TUM1-Iso1, with dithiothreitol as cosubstrate, at pH 10.5 and 37°C Homo sapiens
1.29
-
3-Mercaptopyruvate isoform TUM1-Iso2, with cyanide as cosubstrate, at pH 10.5 and 37°C Homo sapiens
1.33
-
3-Mercaptopyruvate isoform TUM1-Iso1, with cyanide as cosubstrate, at pH 10.5 and 37°C Homo sapiens
2.59
-
dithiothreitol isoform TUM1-Iso2, with dithiothreitol as cosubstrate, at pH 10.5 and 37°C Homo sapiens
2.69
-
dithiothreitol isoform TUM1-Iso1, with dithiothreitol as cosubstrate, at pH 10.5 and 37°C Homo sapiens
4.45
-
cyanide isoform TUM1-Iso1, with cyanide as cosubstrate, at pH 10.5 and 37°C Homo sapiens
4.5
-
cyanide isoform TUM1-Iso2, with cyanide as cosubstrate, at pH 10.5 and 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol isoform TUM1-Iso1 and TUM1-Iso2 Homo sapiens 5829
-
mitochondrion isoforms TUM1-Iso2 Homo sapiens 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
x * 25000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-mercaptopyruvate + cyanide Homo sapiens
-
pyruvate + thiocyanate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P25325
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-agarose column chromatography and benzamidine Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-mercaptopyruvate + cyanide
-
Homo sapiens pyruvate + thiocyanate
-
?
3-mercaptopyruvate + dithiothreitol
-
Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
3-mercaptopyruvate:cyanide sulfurtransferase
-
Homo sapiens
MPST
-
Homo sapiens
TUM1
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
49
-
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.6
-
3-Mercaptopyruvate isoform TUM1-Iso2, with cyanide as cosubstrate, at pH 10.5 and 37°C Homo sapiens
8.8
-
3-Mercaptopyruvate isoform TUM1-Iso1, with cyanide as cosubstrate, at pH 10.5 and 37°C Homo sapiens
20.2
-
3-Mercaptopyruvate isoform TUM1-Iso2, with dithiothreitol as cosubstrate, at pH 10.5 and 37°C Homo sapiens
20.9
-
3-Mercaptopyruvate isoform TUM1-Iso1, with dithiothreitol as cosubstrate, at pH 10.5 and 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
Homo sapiens

General Information

General Information Comment Organism
metabolism the enzyme interacts with proteins involved in Moco and FeS cluster biosynthesis like L-cysteine desulfurase NFS1 and the rhodanese-like protein MOCS3 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.9
-
cyanide isoform TUM1-Iso2, with cyanide as cosubstrate, at pH 10.5 and 37°C Homo sapiens
2
-
cyanide isoform TUM1-Iso1, with cyanide as cosubstrate, at pH 10.5 and 37°C Homo sapiens
6.6
-
3-Mercaptopyruvate isoform TUM1-Iso1, with cyanide as cosubstrate, at pH 10.5 and 37°C Homo sapiens
6.7
-
3-Mercaptopyruvate isoform TUM1-Iso2, with cyanide as cosubstrate, at pH 10.5 and 37°C Homo sapiens
7.7
-
dithiothreitol isoform TUM1-Iso2, with dithiothreitol as cosubstrate, at pH 10.5 and 37°C Homo sapiens
7.8
-
dithiothreitol isoform TUM1-Iso1, with dithiothreitol as cosubstrate, at pH 10.5 and 37°C Homo sapiens
38.2
-
3-Mercaptopyruvate isoform TUM1-Iso1, with dithiothreitol as cosubstrate, at pH 10.5 and 37°C Homo sapiens
38.2
-
3-Mercaptopyruvate isoform TUM1-Iso2, with dithiothreitol as cosubstrate, at pH 10.5 and 37°C Homo sapiens