Cloned (Comment) | Organism |
---|---|
- |
Leishmania mexicana |
wild-type and 4 truncated versions lacking 10, 32, 50 and 70 amino acids at the C-terminus | Leishmania major |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
3-Mercaptopyruvate | pH 7.3, 37°C | Leishmania major | |
0.3 | - |
reduced thioredoxin | pH 7.3, 37°C | Leishmania major | |
1.7 | - |
thiosulfate | pH 7.3, 37°C | Leishmania major |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Leishmania major | 5829 | - |
cytosol | - |
Leishmania mexicana | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
SDS-PAGE | Leishmania major |
40000 | - |
SDS-PAGE | Leishmania mexicana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopyruvate + cyanide | Leishmania major | - |
pyruvate + thiocyanate | - |
? | |
3-mercaptopyruvate + cyanide | Leishmania mexicana | - |
pyruvate + thiocyanate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | Q7K9G0 | - |
- |
Leishmania mexicana | Q8T381 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Leishmania mexicana |
wild-type and 4 truncated versions lacking 10, 32, 50 and 70 amino acids at the C-terminus | Leishmania major |
Renatured (Comment) | Organism |
---|---|
enzyme regains full activity without the need for assistance in the form of a chaperone or detergent after denaturation is 6 M urea and renaturation using dialysis or dilution | Leishmania major |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | - |
Leishmania major | - |
promastigote | - |
Leishmania major | - |
promastigote | - |
Leishmania mexicana | - |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 12 months without loss of activity | Leishmania major |
-20°C, stable for at least 12 months without loss of activity | Leishmania mexicana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopyruvate + cyanide | - |
Leishmania major | pyruvate + thiocyanate | - |
? | |
3-mercaptopyruvate + cyanide | - |
Leishmania mexicana | pyruvate + thiocyanate | - |
? | |
sulfane sulfur + cyanide | - |
Leishmania major | thiocyanate + ? | - |
? | |
sulfane sulfur + cyanide | - |
Leishmania mexicana | thiocyanate + ? | - |
? | |
thiosulfate + thioredoxin | - |
Leishmania major | ? | - |
? | |
thiosulfate + thioredoxin | - |
Leishmania mexicana | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | 7.6 | - |
Leishmania major |
6.9 | 7.6 | - |
Leishmania mexicana |