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Literature summary for 2.8.1.2 extracted from

  • Williams, R.A.; Kelly, S.M.; Mottram, J.C.; Coombs, G.H.
    3-Mercaptopyruvate sulfurtransferase of Leishmania contains an unusual C-terminal extension and is involved in thioredoxin and antioxidant metabolism (2003), J. Biol. Chem., 278, 1480-1486.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Leishmania mexicana
wild-type and 4 truncated versions lacking 10, 32, 50 and 70 amino acids at the C-terminus Leishmania major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
3-Mercaptopyruvate pH 7.3, 37°C Leishmania major
0.3
-
reduced thioredoxin pH 7.3, 37°C Leishmania major
1.7
-
thiosulfate pH 7.3, 37°C Leishmania major

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Leishmania major 5829
-
cytosol
-
Leishmania mexicana 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
SDS-PAGE Leishmania major
40000
-
SDS-PAGE Leishmania mexicana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-mercaptopyruvate + cyanide Leishmania major
-
pyruvate + thiocyanate
-
?
3-mercaptopyruvate + cyanide Leishmania mexicana
-
pyruvate + thiocyanate
-
?

Organism

Organism UniProt Comment Textmining
Leishmania major Q7K9G0
-
-
Leishmania mexicana Q8T381
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Leishmania mexicana
wild-type and 4 truncated versions lacking 10, 32, 50 and 70 amino acids at the C-terminus Leishmania major

Renatured (Commentary)

Renatured (Comment) Organism
enzyme regains full activity without the need for assistance in the form of a chaperone or detergent after denaturation is 6 M urea and renaturation using dialysis or dilution Leishmania major

Source Tissue

Source Tissue Comment Organism Textmining
amastigote
-
Leishmania major
-
promastigote
-
Leishmania major
-
promastigote
-
Leishmania mexicana
-

Storage Stability

Storage Stability Organism
-20°C, stable for at least 12 months without loss of activity Leishmania major
-20°C, stable for at least 12 months without loss of activity Leishmania mexicana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-mercaptopyruvate + cyanide
-
Leishmania major pyruvate + thiocyanate
-
?
3-mercaptopyruvate + cyanide
-
Leishmania mexicana pyruvate + thiocyanate
-
?
sulfane sulfur + cyanide
-
Leishmania major thiocyanate + ?
-
?
sulfane sulfur + cyanide
-
Leishmania mexicana thiocyanate + ?
-
?
thiosulfate + thioredoxin
-
Leishmania major ?
-
?
thiosulfate + thioredoxin
-
Leishmania mexicana ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9 7.6
-
Leishmania major
6.9 7.6
-
Leishmania mexicana