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Literature summary for 2.8.1.2 extracted from
- Purification and properties of mercaptopyruvate sulfur transferase of Escherichia coli (1972), Biochim. Biophys. Acta, 258, 133-146.
General Stability
| General Stability | Organism |
|---|---|
| dialysis against urea inactivates, effect is reversed by dialysis, dilution or electrophoresis | Escherichia coli |
| markedly stabilized during purification and storage by the presence of monovalent cations, maximal stability is obtained if purification and storage are carried out at pH 6.5-7.5 in presence of 0.8 M KCl and 2 mM 2-mercaptoethanol | Escherichia coli |
| stabilized in 0.8 M KCl | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | high concentrations | Escherichia coli |  |
| cyanide | inhibits at short-time intervals, slight enhancement at longer periods | Escherichia coli | |
| cysteamine | slight | Escherichia coli | |
| cysteine | - |
Escherichia coli | |
| glutathione | - |
Escherichia coli | |
| Mercaptosuccinamic acid | slight | Escherichia coli | |
| pyruvate | 10 mM: 17% inhibition, 20 mM: 45% inhibition | Escherichia coli | |
| thioglycolic acid | slight | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.34 | - |
3-Mercaptopyruvate | determination of pyruvate formation, 37°C, pH 9.55 | Escherichia coli | |
| 12.5 | - |
3-Mercaptopyruvate | determination of thiocyanate formation, 37°C, pH 9.55 | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | contains 0.5 mol copper per mol of protein | Escherichia coli | |
| K2SO4 | 0.02 M, 70% activation | Escherichia coli | |
| KCl | 0.02 M, 70% activation | Escherichia coli | |
| additional information | no effect: 0.02 M CdCl2, 0.5 mM arsenite, 0.01 mM copper acetate | Escherichia coli | |
| Na2SO4 | 0.02 M, 70% activation | Escherichia coli | |
| Zn2+ | zinc protein, 1 atom/mol | Escherichia coli | |
| Zn2+ | no indication of a function in the mechanism of catalysis | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23800 | - |
sedimentation equilibrium ultracentrifugation | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-mercaptopyruvate + cyanide | Escherichia coli | - |
pyruvate + thiocyanate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 540 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, no loss of activity after 10 days | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-mercaptopyruvate + cyanide | - |
Escherichia coli | pyruvate + thiocyanate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
| 45 | 50 | - |
Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 60 | 45-50°C: temperature optimum, 60°C: no activity | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12.5 | - |
3-Mercaptopyruvate | in the presence of cyanide, pH 9.55, 37°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.3 | 9.6 | - |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
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