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Literature summary for 2.8.1.13 extracted from

  • Black, K.A.; Dos Santos, P.C.
    Abbreviated pathway for biosynthesis of 2-thiouridine in Bacillus subtilis (2015), J. Bacteriol., 197, 1952-1962.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
C104A mutation completely hinders the ability of MnmA along with YrvO to synthesize s2U in vivo Bacillus subtilis
C200A mutation completely hinders the ability of MnmA along with YrvO to synthesize s2U in vivo Bacillus subtilis
C51A mutation completely hinders the ability of MnmA along with YrvO to synthesize s2U in vivo Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O35020
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-
Bacillus subtilis 168 O35020
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a [protein]-S-sulfanyl-L-cysteine + uridine34 in tRNA + ATP + reduced acceptor
-
Bacillus subtilis a [protein]-L-cysteine + 2-thiouridine34 in tRNA + AMP + diphosphate + acceptor enzyme adenylates and subsequently thiolates tRNA to form the s2U modification ?
a [protein]-S-sulfanyl-L-cysteine + uridine34 in tRNA + ATP + reduced acceptor
-
Bacillus subtilis 168 a [protein]-L-cysteine + 2-thiouridine34 in tRNA + AMP + diphosphate + acceptor enzyme adenylates and subsequently thiolates tRNA to form the s2U modification ?

Synonyms

Synonyms Comment Organism
MnmA
-
Bacillus subtilis

General Information

General Information Comment Organism
physiological function in a conditional Bacillus subtilis knockout strain s2U abundance correlates with tRNA-uridine 2-sulfurtransferase MnmA expression, and MnmA is able to complement Escherichia coli MnmA-deficient strains. Cysteine desulfurase IscS is able to transfer sulfur directly to MnmA in presence of ATP. Both proteins are sufficient for s2U biosynthesis in a pathway independent of the one used in Escherichia coli Bacillus subtilis