Literature summary for 2.8.1.13 extracted from

Numata, T.; Ikeuchi, Y.; Fukai, S.; Adachi, H.; Matsumura, H.; Takano, K.; Murakami, S.; Inoue, T.; Mori, Y.; Sasaki, T.; Suzuki, T.; Nureki, O.
Crystallization and preliminary X-ray analysis of the tRNA thiolation enzyme MnmA from Escherichia coli complexed with tRNA(Glu) (2006), Acta Crystallogr. Sect. F, 62, 368-371.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapour-diffusion method, the binary complex of MnmA and tRNAGlu is crystallized in two different crystal forms: forms I and II. Cocrystallization of MnmAtRNAGlu with ATP yields form III crystals. The three crystal forms diffracted to 3.1 A, 3.4 A and 3.4 A resolution, respectively	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
TusE-SSH + adenylated-tRNA-uridine	Escherichia coli	MnmA catalyzes a sulfuration reaction to synthesize 2-thiouridine at the wobble positions of tRNAGlu, tRNAGln and tRNALys in Escherichia coli	TusE-SH + tRNA-2-thiouridine + AMP	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P25745	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
TusE-SSH + adenylated-tRNA-uridine	-	Escherichia coli	TusE-SH + tRNA-2-thiouridine + AMP	-	?
TusE-SSH + adenylated-tRNA-uridine	MnmA catalyzes a sulfuration reaction to synthesize 2-thiouridine at the wobble positions of tRNAGlu, tRNAGln and tRNALys in Escherichia coli	Escherichia coli	TusE-SH + tRNA-2-thiouridine + AMP	-	?

Synonyms

Synonyms	Comment	Organism
MnmA	-	Escherichia coli

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Release 2023.1 (January 2023)