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Literature summary for 2.8.1.12 extracted from

  • Unkles, S.E.; Heck, I.S.; Appleyard, M.V.; Kinghorn, J.R.
    Eukaryotic molybdopterin synthase. Biochemical and molecular studies of Aspergillus nidulans cnxG and cnxH mutants (1999), J. Biol. Chem., 274, 19286-19293.
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
additional information isolation of cnxG and cnxH mutants, i.e. mutations cnxG10, cnxG24, cnxG100, cnxG166, cnxH43, cnxH86, cnxH89, cnxH255, and cnxH604, overview. Precursor Z and molybdopterin levels in chlorate-resistant and chlorate-sensitive cnxG and cnxH mutant strains, i.e. strains cnxG4 and cnxH3, and strains cnxG2 and cnxH1, overview Aspergillus nidulans
additional information isolation of cnxG and cnxH mutants, i.e. mutations cnxG10, cnxG24, cnxG100, cnxG166, cnxH43, cnxH86, cnxH89, cnxH255, and cnxH604, overview. Precursor Z and molybdopterin levels in chlorate-resistant and chlorate-sensitive cnxG and cnxH mutant strains, overview. Molybdopterin is undetectable in the chlorate-resistant mutants, cnxG4 and cnxH3, whereas up to 2% and 10% of the wild-type level is found in the chlorate-sensitive strains cnxG2 and cnxH1, respectively. The chlorate-sensitive A. nidulans strain, cnxH1, contains an alteration of the translational stop codon such that it is translated to give the amino acid residue Cys, now residue 196, followed by 31 novel amino acids before the next observed stop codon Aspergillus nidulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
9600
-
 x * 9600, small subunit CnxG, SDS-PAGE Aspergillus nidulans
21600
-
 x * 21600, large sbunit CnxH, SDS-PAGE Aspergillus nidulans

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans Q9Y8C1 gene cnxH encoding the large subunit of MPT synthase; genes cnxG and cnxH, encoding the small subunit and the large subunit, respectively
-
Aspergillus nidulans Q9Y8C2 gene cnxG encoding the small subunit of MPT synthase; genes cnxG and cnxH, encoding the small subunit and the large subunit, respectively
-
no activity in Saccharomyces cerevisiae
-
-
-

Subunits

Subunits Comment Organism
? x * 21600, large sbunit CnxH, SDS-PAGE Aspergillus nidulans
? x * 9600, small subunit CnxG, SDS-PAGE Aspergillus nidulans

Synonyms

Synonyms Comment Organism
CnxG small subunit, gene name Aspergillus nidulans
CnxH large ubunit, gene name Aspergillus nidulans

General Information

General Information Comment Organism
evolution homologues of both molybdopterin synthase subunits are evident in diverse eukaryotic sources such as worm, rat, mouse, rice, and fruit fly as well as humans. In contrast, molybdopterin synthase homologues are absent in the yeast Saccharomyces cerevisiae Aspergillus nidulans
malfunction chlorate-sensitive mutants, all the result of amino acid substitutions, produce low levels of molybdopterin and may have low levels of molybdoenzymes. In contrast, chlorate-resistant cnx strains have undetectable levels of molybdopterin, lack the ability to utilize nitrate or hypoxanthine as sole nitrogen sources, and are probably null mutations. Two independent alterations at residue Gly-148 in the large subunit, CnxH, result in temperature sensitivity, not the thermolabile nitrate reductase Aspergillus nidulans
malfunction chlorate-sensitive mutants, all the result of amino acid substitutions, produce low levels of molybdopterin and may have low levels of molybdoenzymes. In contrast, chlorate-resistant cnx strains have undetectable levels of molybdopterin, lack the ability to utilize nitrate or hypoxanthine as sole nitrogen sources, and are probably null mutations. Two independent alterations at residue Gly-148 in the large subunit, CnxH, result in temperature sensitivity, not the thermolabile nitrate reductase. Molybdopterin is undetectable in the chlorate-resistant mutants, cnxG4 and cnxH3, whereas up to 2% and 10% of the wild-type level is found in the chlorate-sensitive strains cnxG2 and cnxH1, respectively Aspergillus nidulans
additional information the C-terminal Gly of the small subunit CnxG, is crucial for the sulfur transfer process during the formation of molybdopterin, residues essential for function, overview Aspergillus nidulans