A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase
Hazra, A.B.; Han, Y.; Chatterjee, A.; Zhang, Y.; Lai, R.Y.; Ealick, S.E.; Begley, T.P.; J. Am. Chem. Soc. 133, 9311-9319 (2011) 
Data extracted from this reference:
Organism
Organism
UniProt
Commentary
Textmining
Bacillus subtilis
O31618
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
-
715306
Bacillus subtilis
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
ThiG
-
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
-
715306
Bacillus subtilis
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
reaction product of ThiG is a thiazole tautomer (R,Z)-2-(2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate, which is aromatized by tautomerase TenI to yield 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate
Bacillus subtilis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
reaction product of ThiG is a thiazole tautomer (R,Z)-2-(2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate, which is aromatized by tautomerase TenI to yield 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate
Bacillus subtilis
Other publictions for EC 2.8.1.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737721
Zhang
Structural Basis for iron-medi ...
Methanocaldococcus jannaschii, Methanotorris igneus, Saccharomyces cerevisiae
Biochemistry
55
1826-1838
2016
-
-
3
3
-
-
-
-
-
-
-
3
-
3
-
-
3
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
-
3
-
-
-
3
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739358
Li
THI1, a thiamine thiazole synt ...
Arabidopsis thaliana
Plant Physiol.
170
1090-1104
2016
-
-
1
-
-
-
-
-
1
-
-
-
-
6
-
-
1
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
739422
Yu
The thiG gene is required for ...
Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzae ZJ173
PLoS ONE
10
e0134237
2015
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
723976
Rohini
Structural insights on Mycobac ...
Mycobacterium tuberculosis
Appl. Biochem. Biotechnol.
169
1790-1798
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
715306
Hazra
A missing enzyme in thiamin th ...
Bacillus subtilis
J. Am. Chem. Soc.
133
9311-9319
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
716340
Chatterjee
Saccharomyces cerevisiae THI4p ...
Saccharomyces cerevisiae
Nature
478
542-546
2011
-
-
1
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
708874
Hazra
Biosynthesis of the thiamin th ...
Bacillus subtilis
J. Am. Chem. Soc.
131
3225-3229
2009
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715251
Chatterjee
Biosynthesis of the thiamin-th ...
Saccharomyces cerevisiae
J. Am. Chem. Soc.
130
11394-11398
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715486
Kriek
Thiazole synthase from Escheri ...
Escherichia coli
J. Biol. Chem.
282
17413-17423
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714159
Jurgenson
Structural insights into the f ...
Saccharomyces cerevisiae
Biochemistry
45
11061-11070
2006
-
-
1
1
-
-
-
-
-
-
-
-
-
5
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
707468
Settembre
Thiamin biosynthesis in Bacill ...
Bacillus subtilis
Biochemistry
43
11647-11657
2004
-
-
1
1
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715242
Dorrestein
The biosynthesis of the thiazo ...
Bacillus subtilis
J. Am. Chem. Soc.
126
3091-3096
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715465
Leonardi
Thiamine biosynthesis in Esche ...
Escherichia coli
J. Biol. Chem.
279
17054-17062
2004
2
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
707466
Park
Biosynthesis of the thiazole m ...
Bacillus subtilis
Biochemistry
42
12430-12438
2003
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-