BRENDA - Enzyme Database
show all sequences of 2.8.1.10

Biosynthesis of the thiamin thiazole in Bacillus subtilis: identification of the product of the thiazole synthase-catalyzed reaction

Hazra, A.; Chatterjee, A.; Begley, T.P.; J. Am. Chem. Soc. 131, 3225-3229 (2009) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Engineering
Protein Variants
Commentary
Organism
additional information
overexpression of His-tagged proteins ThiF, NifS, ThiO, and ThiSG Escherichia coli BL21and incubation with L-cysteine in the presence of dithiothreitol and ATP to form ThiS-COSH. This is then added to 1-deoxy-D-xylulose 5-phosphate and glycine in the presence of ThiO and ThiG to produce the product of the thiazole synthase-catalyzed reaction. Reaction product is a dearomatized thiazole tautomer
Bacillus subtilis
Organism
Organism
UniProt
Commentary
Textmining
Bacillus subtilis
O31618
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-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
-
708874
Bacillus subtilis
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
product is a dearomatized thiazole tautomer, not thiazole phosphate
-
-
?
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
overexpression of His-tagged proteins ThiF, NifS, ThiO, and ThiSG Escherichia coli BL21and incubation with L-cysteine in the presence of dithiothreitol and ATP to form ThiS-COSH. This is then added to 1-deoxy-D-xylulose 5-phosphate and glycine in the presence of ThiO and ThiG to produce the product of the thiazole synthase-catalyzed reaction. Reaction product is a dearomatized thiazole tautomer
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
-
708874
Bacillus subtilis
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
product is a dearomatized thiazole tautomer, not thiazole phosphate
-
-
?
Other publictions for EC 2.8.1.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737721
Zhang
Structural Basis for iron-medi ...
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1826-1838
2016
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739358
Li
THI1, a thiamine thiazole synt ...
Arabidopsis thaliana
Plant Physiol.
170
1090-1104
2016
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739422
Yu
The thiG gene is required for ...
Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzae ZJ173
PLoS ONE
10
e0134237
2015
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723976
Rohini
Structural insights on Mycobac ...
Mycobacterium tuberculosis
Appl. Biochem. Biotechnol.
169
1790-1798
2013
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715306
Hazra
A missing enzyme in thiamin th ...
Bacillus subtilis
J. Am. Chem. Soc.
133
9311-9319
2011
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716340
Chatterjee
Saccharomyces cerevisiae THI4p ...
Saccharomyces cerevisiae
Nature
478
542-546
2011
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6
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708874
Hazra
Biosynthesis of the thiamin th ...
Bacillus subtilis
J. Am. Chem. Soc.
131
3225-3229
2009
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715251
Chatterjee
Biosynthesis of the thiamin-th ...
Saccharomyces cerevisiae
J. Am. Chem. Soc.
130
11394-11398
2008
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4
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715486
Kriek
Thiazole synthase from Escheri ...
Escherichia coli
J. Biol. Chem.
282
17413-17423
2007
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714159
Jurgenson
Structural insights into the f ...
Saccharomyces cerevisiae
Biochemistry
45
11061-11070
2006
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1
1
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5
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707468
Settembre
Thiamin biosynthesis in Bacill ...
Bacillus subtilis
Biochemistry
43
11647-11657
2004
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715242
Dorrestein
The biosynthesis of the thiazo ...
Bacillus subtilis
J. Am. Chem. Soc.
126
3091-3096
2004
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715465
Leonardi
Thiamine biosynthesis in Esche ...
Escherichia coli
J. Biol. Chem.
279
17054-17062
2004
2
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707466
Park
Biosynthesis of the thiazole m ...
Bacillus subtilis
Biochemistry
42
12430-12438
2003
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