Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1)
Park, J.H.; Dorrestein, P.C.; Zhai, H.; Kinsland, C.; McLafferty, F.W.; Begley, T.P.; Biochemistry 42, 12430-12438 (2003) 
Data extracted from this reference:
Application
Application
Commentary
Organism
synthesis
synthesis of the thiazole moiety of thiamin from glycine, cysteine, and deoxy-D-xylulose-5-phosphate using overexpressed Bacillus subtilis ThiF, ThiS, ThiO, ThiG, and a NifS-like protein
Bacillus subtilis
Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Bacillus subtilis
Organism
Organism
UniProt
Commentary
Textmining
Bacillus subtilis
O31618
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-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
ThiG alone can catalyze the formation of thiazole phosphate
707466
Bacillus subtilis
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
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-
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?
Synonyms
Synonyms
Commentary
Organism
ThiG
-
Bacillus subtilis
Application (protein specific)
Application
Commentary
Organism
synthesis
synthesis of the thiazole moiety of thiamin from glycine, cysteine, and deoxy-D-xylulose-5-phosphate using overexpressed Bacillus subtilis ThiF, ThiS, ThiO, ThiG, and a NifS-like protein
Bacillus subtilis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
ThiG alone can catalyze the formation of thiazole phosphate
707466
Bacillus subtilis
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
biochemical function of each of the proteins involved in biosynthesis of thiamin diphosphate is as follws: ThiF catalyzes the adenylation of ThiS, NifS catalyzes the transfer of sulfur from cysteine to the acyl adenylate of ThiS, ThiO catalyzes the oxidation of glycine to the corresponding imine, and ThiG catalyzes the formation of the thiazole phosphate ring
Bacillus subtilis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
biochemical function of each of the proteins involved in biosynthesis of thiamin diphosphate is as follws: ThiF catalyzes the adenylation of ThiS, NifS catalyzes the transfer of sulfur from cysteine to the acyl adenylate of ThiS, ThiO catalyzes the oxidation of glycine to the corresponding imine, and ThiG catalyzes the formation of the thiazole phosphate ring
Bacillus subtilis
Other publictions for EC 2.8.1.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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715306
Hazra
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J. Am. Chem. Soc.
133
9311-9319
2011
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Nature
478
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2011
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708874
Hazra
Biosynthesis of the thiamin th ...
Bacillus subtilis
J. Am. Chem. Soc.
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2009
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715251
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2008
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715486
Kriek
Thiazole synthase from Escheri ...
Escherichia coli
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2007
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714159
Jurgenson
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45
11061-11070
2006
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707468
Settembre
Thiamin biosynthesis in Bacill ...
Bacillus subtilis
Biochemistry
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2004
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715242
Dorrestein
The biosynthesis of the thiazo ...
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J. Am. Chem. Soc.
126
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2004
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715465
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Thiamine biosynthesis in Esche ...
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707466
Park
Biosynthesis of the thiazole m ...
Bacillus subtilis
Biochemistry
42
12430-12438
2003
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