Any feedback?
Literature summary for 2.8.1.1 extracted from
- Cyanide detoxification by recombinant bacterial rhodanese (2005), Chemosphere, 63, 942-949.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | expression of enzyme in Escherichia coli results in increased resistance to 5 mM cyanide | Pseudomonas aeruginosa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | about 95% of enzyme | Pseudomonas aeruginosa | 5737 | - |
| periplasm | about 5% of enzyme | Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
x * 32000, SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiosulfate + cyanide | Pseudomonas aeruginosa | cyanide detoxification by engineered Escherichia coli is feasible under laboratory conditions and suggests that microbial rhodaneses may contribute to cyanide transformation in natural environments | sulfite + thiocyanate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
| Pseudomonas aeruginosa | - |
expression in Escherichia coli | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 816 | - |
pH 7.3, 25°C | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiosulfate + cyanide | - |
Pseudomonas aeruginosa | sulfite + thiocyanate | - |
? | |
| thiosulfate + cyanide | cyanide detoxification by engineered Escherichia coli is feasible under laboratory conditions and suggests that microbial rhodaneses may contribute to cyanide transformation in natural environments | Pseudomonas aeruginosa | sulfite + thiocyanate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| r-RhdA | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 32 | broad | Pseudomonas aeruginosa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
