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Literature summary for 2.8.1.1 extracted from

  • Lee, C.Y.; Hwang, J.H.; Lee, Y.S.; Cho, K.S.
    Purification and characterization of mouse liver rhodanese (1995), J. Biochem. Mol. Biol., 28, 170-176.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ more than 70% inhibition at 0.1 mM Mus musculus
Cu2+ more than 70% inhibition at 0.1 mM Mus musculus
Hg2+ more than 70% inhibition at 0.1 mM Mus musculus
Ni2+ more than 70% inhibition at 0.1 mM Mus musculus
Zn2+ more than 70% inhibition at 0.1 mM Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.3
-
thiosulfate 25°C, pH 9.4 Mus musculus
12.5
-
CN- 25°C, pH 9.4 Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
1 * 34000, SDS-PAGE Mus musculus
34800
-
gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thiosulfate + cyanide Mus musculus
-
sulfite + thiocyanate
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1071
-
after Sephacryl S-200-HR gel filtration Mus musculus

Storage Stability

Storage Stability Organism
-70°C, 3 weeks, enzyme splits in two identical subunits with a molecular weight of 17000 Da Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thiosulfate + cyanide
-
Mus musculus sulfite + thiocyanate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 34000, SDS-PAGE Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Mus musculus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
50
-
Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.4
-
-
Mus musculus

pI Value

Organism Comment pI Value Maximum pI Value
Mus musculus isoelectric focusing in Phast Gel isoelectric focusing gel with pH range: 3 - 9
-
6.6