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Literature summary for 2.8.1.1 extracted from

  • Luo, G.X.; Horowitz, P.M.
    The sulfurtransferase activity and structure of rhodanese are affected by site-directed replacement of Arg-186 or Lys-249 (1994), J. Biol. Chem., 269, 8220-8225.
    View publication on PubMed
Search for more literature for 2.8.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bos taurus

Protein Variants

Protein Variants Comment Organism
K249A reduced stability, comparable secondary structure, more easily exposed hydrophobic surfaces and a core structure that denatured similarly to the wild-type enzyme, inactive using thiosulfate, but active with thiosulfonates Bos taurus
R186L increased KM for thiosulfate Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7
-
 thiosulfate pH 7.5, wild-type Bos taurus
73.2
-
 thiosulfate pH 7.5, R186L Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thiosulfate + cyanide Bos taurus
-
 sulfite + thiocyanate
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
refolds from 8 M urea to enzymatically active species Bos taurus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
575.5
-
 R186L Bos taurus
672.8
-
 wild-type Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyanide + 2-aminoethanethiosulfate K249A Bos taurus ?
-
 ?
cyanide + ethanethiosulfate K249A Bos taurus ?
-
 ?
cyanide + p-toluenethiosulfonate K249A Bos taurus ?
-
 ?
thiosulfate + cyanide
-
 Bos taurus sulfite + thiocyanate
-
 ?