Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli M15 | Azotobacter vinelandii |
Protein Variants | Comment | Organism |
---|---|---|
K249A | complete inactivation of rhodanese with thiosulfate | Bos taurus |
T232A | increase in thiosulfate-cyanide sulfurtransferase activity and no activity with 3-mercaptopyruvate as sulfur donor | Azotobacter vinelandii |
T232K | increase in thiosulfate-cyanide sulfurtransferase activity and no activity with 3-mercaptopyruvate as sulfur donor | Azotobacter vinelandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiosulfate + cyanide | Bos taurus | - |
sulfite + thiocyanate | - |
? | |
thiosulfate + cyanide | Azotobacter vinelandii | - |
sulfite + thiocyanate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Azotobacter vinelandii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
225 | - |
recombinant wild-type enzyme | Azotobacter vinelandii |
440 | - |
T232A | Azotobacter vinelandii |
550 | - |
T232K | Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiosulfate + cyanide | - |
Bos taurus | sulfite + thiocyanate | - |
? | |
thiosulfate + cyanide | - |
Azotobacter vinelandii | sulfite + thiocyanate | - |
? |