BRENDA - Enzyme Database show
show all sequences of 2.8.1.1

Purification and characterization of rhodanese from Acinetobacter calcoaceticus

Vandenbergh, P.A.; Berk, R.S.; Can. J. Microbiol. 26, 281-286 (1980)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
enhances activity
Acinetobacter calcoaceticus
L-cysteine
enhances activity
Acinetobacter calcoaceticus
reduced glutathione
enhances activity
Acinetobacter calcoaceticus
Inhibitors
Inhibitors
Commentary
Organism
Structure
iodoacetate
-
Acinetobacter calcoaceticus
NEM
-
Acinetobacter calcoaceticus
p-chloromercuribenzoate
10 mM, 5% inhibition
Acinetobacter calcoaceticus
SDS
-
Acinetobacter calcoaceticus
SO32-
-
Acinetobacter calcoaceticus
Triton X-100
slight
Acinetobacter calcoaceticus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Acinetobacter calcoaceticus
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
gel filtration
Acinetobacter calcoaceticus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
thiosulfate + cyanide
Acinetobacter calcoaceticus
-
sulfite + thiocyanate
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acinetobacter calcoaceticus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Acinetobacter calcoaceticus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
70
-
QAE-Sephadex column II
Acinetobacter calcoaceticus
Storage Stability
Storage Stability
Organism
-20°C, several months
Acinetobacter calcoaceticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
thiosulfate + cyanide
-
645515
Acinetobacter calcoaceticus
sulfite + thiocyanate
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 35000, SDS-PAGE
Acinetobacter calcoaceticus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Acinetobacter calcoaceticus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
rapid denaturation above
Acinetobacter calcoaceticus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
8.5
-
Acinetobacter calcoaceticus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
enhances activity
Acinetobacter calcoaceticus
L-cysteine
enhances activity
Acinetobacter calcoaceticus
reduced glutathione
enhances activity
Acinetobacter calcoaceticus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodoacetate
-
Acinetobacter calcoaceticus
NEM
-
Acinetobacter calcoaceticus
p-chloromercuribenzoate
10 mM, 5% inhibition
Acinetobacter calcoaceticus
SDS
-
Acinetobacter calcoaceticus
SO32-
-
Acinetobacter calcoaceticus
Triton X-100
slight
Acinetobacter calcoaceticus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Acinetobacter calcoaceticus
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
gel filtration
Acinetobacter calcoaceticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
thiosulfate + cyanide
Acinetobacter calcoaceticus
-
sulfite + thiocyanate
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Acinetobacter calcoaceticus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
70
-
QAE-Sephadex column II
Acinetobacter calcoaceticus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, several months
Acinetobacter calcoaceticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
thiosulfate + cyanide
-
645515
Acinetobacter calcoaceticus
sulfite + thiocyanate
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 35000, SDS-PAGE
Acinetobacter calcoaceticus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Acinetobacter calcoaceticus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
rapid denaturation above
Acinetobacter calcoaceticus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
8.5
-
Acinetobacter calcoaceticus
Other publictions for EC 2.8.1.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739155
Morton
Genetic identification of thio ...
Mus musculus
Nat. Med.
22
771-779
2016
-
1
-
-
-
-
-
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1
-
-
1
-
4
-
-
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1
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1
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1
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1
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1
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-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
737706
Higgins
Conformational analysis and ch ...
Staphylococcus aureus
Biochemistry
54
2385-2398
2015
-
-
-
-
-
-
-
-
-
-
1
1
-
3
-
-
-
-
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2
1
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-
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1
1
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-
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-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
738551
Singh
Dose and time-dependent effect ...
Mus musculus
J. Biochem. Mol. Toxicol.
27
499-507
2013
-
-
-
-
-
-
-
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1
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1
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2
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3
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3
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1
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1
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1
-
-
739474
Wallrodt
The putative thiosulfate sulfu ...
Salmonella enterica
PLoS ONE
8
e70829
2013
-
-
-
-
-
-
-
-
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1
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1
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1
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1
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-
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-
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-
1
1
-
-
-
724480
Giuliani
Characterization of a new peri ...
Aquifex aeolicus
Biochimie
92
388-397
2010
-
-
-
-
-
-
1
2
1
-
3
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
-
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-
1
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2
1
-
3
-
-
-
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-
-
-
-
-
2
1
-
-
-
1
-
-
-
-
1
-
-
1
-
-
724781
Krueger
Low expression of thiosulfate ...
Homo sapiens
Clin. Biochem.
43
95-101
2010
-
1
-
-
-
-
-
-
-
-
1
-
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2
-
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1
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1
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1
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1
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1
-
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-
1
-
-
-
-
-
-
-
-
1
1
1
1
-
-
725406
Smirnov
Mitochondrial enzyme rhodanese ...
Homo sapiens
J. Biol. Chem.
285
30792-30803
2010
-
-
-
-
-
-
-
-
5
-
1
-
-
2
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-
1
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1
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5
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1
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1
-
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-
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
690262
Witholt
Expression, purification, crys ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Acta Crystallogr. Sect. F
64
541-544
2008
-
1
1
1
-
-
-
-
-
-
-
2
-
153
-
-
1
-
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-
4
-
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1
1
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1
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2
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1
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-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671035
Wrobel
Menadione effect on L-cysteine ...
Homo sapiens
Acta Biochim. Pol.
54
407-411
2007
-
-
-
-
-
-
1
-
-
-
-
-
-
1
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1
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1
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1
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-
-
-
-
-
-
-
671493
Cipollone
Involvement of Pseudomonas aer ...
Pseudomonas aeruginosa
Appl. Environ. Microbiol.
73
390-398
2007
-
-
-
-
1
-
-
-
1
-
-
-
-
6
-
-
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1
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1
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672482
Bartels
Conformational studies on Arab ...
Arabidopsis thaliana
Biol. Chem.
388
53-59
2007
-
-
-
-
3
-
-
-
-
-
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1
-
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3
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673175
Aminlari
-
Cyanide-metabolizing enzyme rh ...
Bos taurus, Camelus dromedarius, Canis lupus familiaris, Capra hircus, Equus caballus, Gallus gallus, Homo sapiens, Ovis aries
Comp. Clin. Pathol.
16
47-51
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
56
51
-
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56
51
-
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-
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-
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-
-
673274
Wilson
Differentiation of the roles o ...
Bos taurus
Dig. Dis. Sci.
53
277-283
2007
-
1
-
-
-
-
-
-
-
-
-
1
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3
-
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3
2
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3
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1
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1
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3
2
-
3
-
-
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-
-
-
-
-
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-
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-
-
673600
Giuliani
A new sulfurtransferase from t ...
Aquifex aeolicus
FEBS J.
274
4572-4587
2007
-
-
-
1
-
-
-
4
1
-
3
-
-
4
-
-
-
-
-
-
1
-
3
3
1
1
-
-
1
1
-
-
-
-
-
-
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-
1
-
-
-
-
-
4
1
-
3
-
-
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-
1
-
3
3
1
1
-
-
1
1
-
-
-
-
-
-
-
-
671241
Ramasamy
Sulfide-detoxifying enzymes in ...
Homo sapiens
Am. J. Physiol. Gastrointest. Liver Physiol.
291
G288-G296
2006
2
1
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
2
-
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2
1
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1
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2
-
-
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-
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-
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673103
Sani
Circadian and ultradian (12 h) ...
Mus musculus
Chronobiol. Int.
23
551-563
2006
-
-
-
-
-
-
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3
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2
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2
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675044
Papezova
Determination of cyanide in mi ...
Bos taurus
J. Chromatogr. A
1120
268-272
2006
-
1
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1
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1
1
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1
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1
1
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1
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677018
Cornilescu
Solution structure of a single ...
Arabidopsis thaliana
Protein Sci.
15
2836-2841
2006
-
-
-
1
-
-
-
-
-
-
-
-
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6
-
-
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-
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1
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677206
Wrobel
Rhodanese in mouse brain: regi ...
Mus musculus
Toxicol. Mech. Methods
16
169-172
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
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-
-
2
-
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2
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677214
Billaut-Laden
Evidence for a functional gene ...
Homo sapiens
Toxicology
225
1-11
2006
-
-
-
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3
-
-
3
-
-
-
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3
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1
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3
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3
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1
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661492
Cipollone
Cyanide detoxification by reco ...
Pseudomonas aeruginosa
Chemosphere
63
942-949
2005
-
-
1
-
1
-
-
-
2
-
1
1
-
3
-
-
-
-
-
-
1
-
2
1
1
-
-
-
-
-
-
-
-
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1
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The N-terminal rhodanese domai ...
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Presence of rhodanese in the c ...
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663321
Kaur
Active rhodanese lacking nones ...
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2004
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663433
Wrobel
Sulfurtransferases and cyanide ...
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645521
Spallarossa
Inhibition of Azotobacter vine ...
Azotobacter vinelandii
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2003
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645523
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Azotobacter vinelandii rhodane ...
Azotobacter vinelandii
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645525
Ybarra
Active rhodanese lacking nones ...
Bos taurus
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645530
Nazifi
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Distribution of rhodanese in t ...
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2003
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645541
Ezzi
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Characterization of the rhodan ...
Fusarium oxysporum, Fusarium solani, Trichoderma harzianum
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32
629-634
2003
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645520
Bauer
Identification and characteriz ...
Arabidopsis thaliana
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645533
Adams
PspE (phage-shock protein E) o ...
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645535
Picton
Mucosal protection against sul ...
Bos taurus, Homo sapiens
Gut
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2002
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7
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645529
Kramer
An additional serine residue a ...
Bos taurus
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385
332-337
2001
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645546
Al-Qarawi
Tissue and intracellular distr ...
Bos indicus, Camelus dromedarius, Columbia domesticus, Gallus gallus, Ovis aries
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63-70
2001
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28
27
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645522
Pagani
Mutagenic analysis of Thr-232 ...
Azotobacter vinelandii, Bos taurus
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307-311
2000
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645524
Nandi
Rhodanese as a thioredoxin oxi ...
Bos taurus
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2
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645532
Ray
Characterization of a 12-kilod ...
Escherichia coli
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2000
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2
1
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1
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645537
Wrobel
Rhodanese activity and total s ...
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38
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2000
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6
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Bordo
The crystal structure of a sul ...
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2000
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1
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645526
Trevino
NH2-terminal sequence truncati ...
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274
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1
1
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645545
Gliubich
Structure of sulfur-substitute ...
Bos taurus
Acta Crystallogr. Sect. D
54
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1998
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1
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-
-
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-
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645534
Wrobel
-
Effects of thiazolidine-4(R)-c ...
Mus musculus
Amino Acids
12
309-314
1997
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1
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2
15
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1
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645539
Vazquez
Mitochondrial and cytosolic rh ...
Mus musculus
Cancer Biochem. Biophys.
15
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1997
1
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2
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645540
Ihm
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Thiosulfate sulfurtransferase ...
Rattus norvegicus
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29
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3
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1
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2
41
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1
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Renaturation of rhodanese by t ...
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2
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645531
Trevino
Chinese hamster rhodanese cDNA ...
Bos taurus, Oncorhynchus mykiss
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6
693-699
1995
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1
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2
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Mus musculus
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1
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1
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Isolation and partial purifica ...
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645527
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The sulfurtransferase activity ...
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645528
Miller-Martin
Mutations of noncatalytic sulf ...
Bos taurus
J. Biol. Chem.
269
3423-3428
1994
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645542
Aminlari
The cyanide-metabolizing enzym ...
Canis lupus familiaris, Ovis aries
Toxicol. Appl. Pharmacol.
124
67-71
1994
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645496
Pagani
Properties of Azotobacter vine ...
Azotobacter vinelandii
Biochem. Mol. Biol. Int.
29
595-604
1993
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1
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645508
Miller
Recombinant bovine rhodanese: ...
Bos taurus
Biochim. Biophys. Acta
1121
286-292
1992
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1
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645509
Miller
Expression of cloned bovine ad ...
Bos taurus
J. Biol. Chem.
266
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1991
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645514
Pallini
Cloning and sequence analysis ...
Bos taurus, Gallus gallus, Homo sapiens
Biochem. Biophys. Res. Commun.
180
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1991
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3
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645517
Turkowsky
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Properties of a soluble thiosu ...
Methanosarcina mazei
FEMS Microbiol. Lett.
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4
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645516
Fukumori
-
Purification and some properti ...
Starkeya novella
FEMS Microbiol. Lett.
65
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1989
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645497
Vazquez
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Porphyrin biosynthesis in Rp. ...
Rhodopseudomonas palustris
Int. J. Biochem.
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1987
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645505
Burrous
Chromogenic and fluorigenic su ...
Bos taurus
Methods Enzymol.
143
235-239
1987
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7
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645518
Alexander
Properties of an Escherichia c ...
Escherichia coli
J. Biol. Chem.
262
6595-6604
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3
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1
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645519
Aird
Isolation and characterization ...
Acinetobacter calcoaceticus, Bos taurus
J. Biol. Chem.
262
17327-17335
1987
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2
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9
2
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645498
Wrobel
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Comparison of some molecular a ...
Rana temporaria
Bull. Pol. Acad. Sci. Biol. Sci.
32
303-313
1984
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2
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645499
Van Rensburg
Rhodanese from Cercopithecus a ...
Chlorocebus aethiops
Int. J. Biochem.
16
539-546
1984
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1
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645507
Pagani
The inhibition of rhodanese by ...
Bos taurus
Biochim. Biophys. Acta
742
116-121
1983
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645500
Anosike
A comparison of some biochemic ...
Cavia porcellus, Rattus norvegicus
Indian J. Biochem. Biophys.
19
13-16
1982
2
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6
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2
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2
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2
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2
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645503
Westley
Thiosulfate: cyanide sulfurtra ...
Bos taurus
Methods Enzymol.
77
285-291
1981
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1
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5
1
1
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8
1
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5
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1
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8
1
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645495
Scott
Identity of beta-mercaptopyruv ...
Homo sapiens
Biochem. Biophys. Res. Commun.
97
1334-1338
1980
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1
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1
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645510
Horowitz
Some comparisons between solut ...
Bos taurus
Biochem. Biophys. Res. Commun.
94
419-423
1980
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1
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2
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645515
Vandenbergh
Purification and characterizat ...
Acinetobacter calcoaceticus
Can. J. Microbiol.
26
281-286
1980
3
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6
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2
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6
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1
1
1
1
1
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1
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1
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-
-
-
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645512
Boey
-
Purification of tapioca leaf r ...
Manihot esculenta
Phytochemistry
15
1343-1344
1976
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-
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1
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1
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1
1
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1
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1
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1
1
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645511
Oi
Inhibition of rat liver rhodan ...
Rattus norvegicus
J. Biochem.
78
825-834
1975
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12
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1
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2
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1
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12
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1
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1
1
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645506
Oi
Inhibition of bovine liver rho ...
Bos taurus
J. Biochem.
76
455-458
1974
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1
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1
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2
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1
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2
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1
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645502
Westley
Rhodanese ...
Acidithiobacillus ferrooxidans, Bos taurus, Brassica rapa subsp. rapa, Brassica sp., Calliphoridae, Canis lupus familiaris, Desulfotomaculum nigrificans, Escherichia coli, Felis catus, Homo sapiens, Manihot esculenta, Oryctolagus cuniculus, Petroselinum crispum, Pseudomonas aeruginosa, Spinacia oleracea, Starkeya novella, Thiobacillus denitrificans
Adv. Enzymol. Relat. Areas Mol. Biol.
39
327-368
1973
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17
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17
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27
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158
4
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74
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10
1
3
22
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27
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158
4
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645513
Oi
-
Purification and some properti ...
Trametes sanguinea
Agric. Biol. Chem.
37
629-635
1973
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8
1
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1
1
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3
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1
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1
1
1
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1
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8
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1
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1
1
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1
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1
1
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3
-
-
-
1
-
1
1
1
1
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-
-
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645501
Cannella
Crystalline rhodanese from bee ...
Bos taurus
Ital. J. Biochem.
21
1-7
1972
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1
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1
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1
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1
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1
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1
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645504
Drenth
Crystallographic data for rhod ...
Bos taurus
Biochem. Biophys. Res. Commun.
45
1320-1322
1971
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1
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1
1
1
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2
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1
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1
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1
1
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1
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