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Literature summary for 2.8.1.1 extracted from
- Purification and characterization of rhodanese from Acinetobacter calcoaceticus (1980), Can. J. Microbiol., 26, 281-286.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | enhances activity | Acinetobacter calcoaceticus |  |
| L-cysteine | enhances activity | Acinetobacter calcoaceticus | |
| reduced glutathione | enhances activity | Acinetobacter calcoaceticus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetate | - |
Acinetobacter calcoaceticus | |
| NEM | - |
Acinetobacter calcoaceticus | |
| p-chloromercuribenzoate | 10 mM, 5% inhibition | Acinetobacter calcoaceticus | |
| SDS | - |
Acinetobacter calcoaceticus | |
| SO32- | - |
Acinetobacter calcoaceticus | |
| Triton X-100 | slight | Acinetobacter calcoaceticus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Acinetobacter calcoaceticus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
gel filtration | Acinetobacter calcoaceticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiosulfate + cyanide | Acinetobacter calcoaceticus | - |
sulfite + thiocyanate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter calcoaceticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acinetobacter calcoaceticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 70 | - |
QAE-Sephadex column II | Acinetobacter calcoaceticus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, several months | Acinetobacter calcoaceticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiosulfate + cyanide | - |
Acinetobacter calcoaceticus | sulfite + thiocyanate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 35000, SDS-PAGE | Acinetobacter calcoaceticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Acinetobacter calcoaceticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
rapid denaturation above | Acinetobacter calcoaceticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Acinetobacter calcoaceticus |
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