Literature summary for 2.7.9.6 extracted from

Qi, X.; Lin, W.; Ma, M.; Wang, C.; He, Y.; He, N.; Gao, J.; Zhou, H.; Xiao, Y.; Wang, Y.; Zhang, P.
Structural basis of rifampin inactivation by rifampin phosphotransferase (2016), Proc. Natl. Acad. Sci. USA, 113, 3803-3808 .

Search for more literature for 2.7.9.6

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Listeria monocytogenes

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 8% (w/v) PEG3350 and 100 mM Bicine (pH 9.2)	Listeria monocytogenes

Protein Variants

Protein Variants	Comment	Organism
G527A	the mutant has much decreased rifampin-phosphorylation activity in vitro and reduced rifampin resistance in vivo	Listeria monocytogenes
G527S	the mutant has much decreased rifampin-phosphorylation activity in vitro and reduced rifampin resistance in vivo	Listeria monocytogenes
G527Y	the mutant has much decreased rifampin-phosphorylation activity in vitro and reduced rifampin resistance in vivo	Listeria monocytogenes
H825A	the mutant loses the ability to phosphorylate rifampin both in vitro and in vivo	Listeria monocytogenes
K670A	the mutant loses the ability to phosphorylate rifampin both in vitro and in vivo	Listeria monocytogenes
Q337A	the mutant has significantly reduced activity compared to the wild type enzyme	Listeria monocytogenes
R666A	the mutant loses the ability to phosphorylate rifampin both in vitro and in vivo	Listeria monocytogenes

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Listeria monocytogenes

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + rifampicin + H2O	Listeria monocytogenes	-	AMP + 21-phosphorifampicin + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Listeria monocytogenes	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex 200 gel filtration	Listeria monocytogenes

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + rifampicin + H2O	-	Listeria monocytogenes	AMP + 21-phosphorifampicin + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
rifampin phosphotransferase	-	Listeria monocytogenes
RPH	-	Listeria monocytogenes

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Release 2023.1 (January 2023)