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Literature summary for 2.7.9.4 extracted from
- A CBM20 low-affinity starch-binding domain from glucan, water dikinase (2009), FEBS Lett., 583, 1159-1163.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the starch-binding domain, SBD, Asp68-Gly184, of glucan, water dikinase 3 is cloned into the vector pET-28a+ for expression in Escherichia coli BL21DE3 cells, the region encoding residues 1-201, containing the chloroplast transit peptide and the SBD is fused to YFP and transiently expressed in Nicotiana benthamiana | Arabidopsis thaliana |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14200 | - |
theoretical, starch-binding domain, SBD, Asp68-Gly184, verified by SDS-PAGE | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Arabidopsis thaliana | GWD3-SBD shows strongest binding towards alpha-cyclodextrin and no detectable affinity for linear maltooligosaccharides or for phosphorylated beta-cyclodextrin | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GWD3-SBD is purified on a beta-cyclodextrin Sepharose column and by an additional His-trap purification step | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | GWD3-SBD shows strongest binding towards alpha-cyclodextrin and no detectable affinity for linear maltooligosaccharides or for phosphorylated beta-cyclodextrin | Arabidopsis thaliana | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucan, water dikinase | - |
Arabidopsis thaliana |
| glucan, water dikinase 3 | - |
Arabidopsis thaliana |
| GWD3 | - |
Arabidopsis thaliana |
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