Cloned (Comment) | Organism |
---|---|
gene ppdk, Triticum aestivum cv. Zhoumai19 immature embryosare transfected by particle bombardment with chimeric genes containing pepc or ppdk cDNA and the bialaphos resistance gene under the control of the Cauliflower mosaic virus 35S promoter. Three types of transgenic lines containing 1. pepc cDNA (PC lines), 2. ppdk cDNA (PK lines), or 3. both genes (PKC lines) are obtained through callus differentiation, phosphinthricine resistance screening, plantlet regeneration, and molecular detection. Quantitative real-time PCR enzyme expression analysis. Net photosynthetic rates of mutants are increased compared to controls | Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
105000 | - |
- |
Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + phosphate | Zea mays | - |
AMP + phosphoenolpyruvate + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | D3K3U9 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + phosphate | - |
Zea mays | AMP + phosphoenolpyruvate + diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 105000, recombinant enzyme PPDK, SDS-PAGE | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
PPDK | - |
Zea mays |
pyruvate orthophosphate dikinase | - |
Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Zea mays | |
ATP | - |
Zea mays |
General Information | Comment | Organism |
---|---|---|
physiological function | pyramiding expression of Zea mays genes encoding phosphoenolpyruvate carboxylase (PEPC) and pyruvate orthophosphate dikinase (PPDK) synergistically improve the photosynthetic characteristics of transgenic Triticum aestivum | Zea mays |