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Literature summary for 2.7.9.1 extracted from

  • Zhang, H.; Xu, W.; Wang, H.; Hu, L.; Li, Y.; Qi, X.; Zhang, L.; Li, C.; Hua, X.
    Pyramiding expression of maize genes encoding phosphoenolpyruvate carboxylase (PEPC) and pyruvate orthophosphate dikinase (PPDK) synergistically improve the photosynthetic characteristics of transgenic wheat (2014), Protoplasma, 251, 1163-1173.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene ppdk, Triticum aestivum cv. Zhoumai19 immature embryosare transfected by particle bombardment with chimeric genes containing pepc or ppdk cDNA and the bialaphos resistance gene under the control of the Cauliflower mosaic virus 35S promoter. Three types of transgenic lines containing 1. pepc cDNA (PC lines), 2. ppdk cDNA (PK lines), or 3. both genes (PKC lines) are obtained through callus differentiation, phosphinthricine resistance screening, plantlet regeneration, and molecular detection. Quantitative real-time PCR enzyme expression analysis. Net photosynthetic rates of mutants are increased compared to controls Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000
-
-
Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pyruvate + phosphate Zea mays
-
AMP + phosphoenolpyruvate + diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Zea mays D3K3U9
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + phosphate
-
Zea mays AMP + phosphoenolpyruvate + diphosphate
-
r

Subunits

Subunits Comment Organism
? x * 105000, recombinant enzyme PPDK, SDS-PAGE Zea mays

Synonyms

Synonyms Comment Organism
PPDK
-
Zea mays
pyruvate orthophosphate dikinase
-
Zea mays

Cofactor

Cofactor Comment Organism Structure
AMP
-
Zea mays
ATP
-
Zea mays

General Information

General Information Comment Organism
physiological function pyramiding expression of Zea mays genes encoding phosphoenolpyruvate carboxylase (PEPC) and pyruvate orthophosphate dikinase (PPDK) synergistically improve the photosynthetic characteristics of transgenic Triticum aestivum Zea mays