Literature summary for 2.7.9.1 extracted from

Famiani, F.; Paoletti, A.; Battistelli, A.; Moscatello, S.; Chen, Z.H.; Leegood, R.C.; Walker, R.P.
Phosphoenolpyruvate carboxykinase, pyruvate orthophosphate dikinase and isocitrate lyase in both tomato fruits and leaves, and in the flesh of peach and some other fruits (2016), J. Plant Physiol., 202, 34-44.

Search for more literature for 2.7.9.1

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Zea mays	5737	-
cytoplasm	-	Solanum lycopersicum	5737	-
cytoplasm	-	Capsicum annuum	5737	-
cytoplasm	-	Hoya carnosa	5737	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
95000	-	x * 95000, SDS-PAGE	Zea mays
95000	-	x * 95000, SDS-PAGE	Solanum lycopersicum
95000	-	x * 95000, SDS-PAGE	Hoya carnosa

Organism

Organism	UniProt	Comment	Textmining
Capsicum annuum	-	cv. World Beater	-
Hoya carnosa	-	-	-
no activity in Prunus persica var. Adriatica	-	-	-
no activity in Solanum melongena var. Black Enorma	-	-	-
Solanum lycopersicum	-	cv. Moneymaker	-
Zea mays	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	in the darkened leaves of C3 plants PPDK is present largely as an inactive phosphorylated form	Solanum lycopersicum

Source Tissue

Source Tissue	Comment	Organism	Textmining
fruit	fruit wall of green and red pepper, low enzyme content	Capsicum annuum	-
fruit	ripe fruit flesh, the enzyme is located in skin, flesh, locular gel and columella of tomato fruits. Its abundance does not increase during ripening, but decreases during senescence	Solanum lycopersicum	-
leaf	mature leaves, high enzyme content	Zea mays	-
leaf	mature leaves, high enzyme content	Hoya carnosa	-
leaf	the content of PPDK g/fresh weight increases in tomato leaves as they mature until the mid-stage of development, and declines during their senescence	Solanum lycopersicum	-
additional information	PPDK polypeptide abundance and not PPDK activity is determined, because in the darkened leaves of C3 plants, PPDK is present largely as an inactive phosphorylated form	Solanum lycopersicum	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0006	-	leaf enzyme extract, pH and temperature not specified in the publication	Solanum lycopersicum
0.1	0.19	leaf enzyme extract, pH and temperature not specified in the publication	Zea mays

Subunits

Subunits	Comment	Organism
?	x * 95000, SDS-PAGE	Zea mays
?	x * 95000, SDS-PAGE	Solanum lycopersicum
?	x * 95000, SDS-PAGE	Hoya carnosa

Synonyms

Synonyms	Comment	Organism
PPDK	-	Zea mays
PPDK	-	Solanum lycopersicum
PPDK	-	Capsicum annuum
PPDK	-	Hoya carnosa
pyruvate orthophosphate dikinase	-	Zea mays
pyruvate orthophosphate dikinase	-	Solanum lycopersicum
pyruvate orthophosphate dikinase	-	Capsicum annuum
pyruvate orthophosphate dikinase	-	Hoya carnosa

General Information

General Information	Comment	Organism
metabolism	the enzyme is involved in gluconeogenesis	Zea mays
metabolism	the enzyme is involved in gluconeogenesis	Hoya carnosa
metabolism	the enzyme is involved in gluconeogenesis. In fruits from other plants than tomato, the bulk of any gluconeogenic flux proceeds via phosphoenolpyruvate carboxykinase, PEPCK	Capsicum annuum
metabolism	the enzyme is involved in gluconeogenesis. In fruits from other plants than tomato, the bulk of any gluconeogenic flux proceeds via phosphoenolpyruvate carboxykinase, PEPCK, whereas in tomato both PEPCK and PPDK can potentially be utilised. The conversion of pyruvate/acetyl-CoA to malate by the glyoxylate cycle, for which glyoxysomal isocitrate lyase is necessary, is not a major pathway utilised by gluconeogenesis in fruits under normal conditions of growth	Solanum lycopersicum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)