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Literature summary for 2.7.9.1 extracted from

  • Rodriguez-Contreras, D.; Hamilton, N.
    Gluconeogenesis in Leishmania mexicana: contribution of glycerol kinase, phosphoenolpyruvate carboxykinase, and pyruvate phosphate dikinase (2014), J. Biol. Chem., 289, 32989-33000.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.9.1

Cloned(Commentary)

Cloned (Comment) Organism
single copy gene LmxM11.1000, the LmxPPDK ORF is subcloned into pX63NeoRI, recombinant expression of N-terminally His6-tagged enzyme Leishmania mexicana

Protein Variants

Protein Variants Comment Organism
additional information generation of a DELTAppdk null mutant by recombinant homologous gene replacement with hygromycin B and phleomycin resistance markers. No difference in growth is observed between PPDK wild-type and null mutant parasite promastigotes, but the incorporation of precursors into storage compound mannogen changes in the mutant compared to wild-type, DELTAppdk promastigotes are able to incorporate 14C label into mannogen when they are incubated with alanine, glycerol, and aspartate. The label incorporation in wild-type and DELTAppdk promastigotes incubated with alanine is 98.6% and 78.9%, respectively Leishmania mexicana

Organism

Organism UniProt Comment Textmining
Leishmania mexicana
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 strain MHOM/GT/2001/U1103
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Leishmania mexicana MNYZ/BZ/62/M379
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 strain MHOM/GT/2001/U1103
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged enzyme by nickel affinity chromatography Leishmania mexicana

Source Tissue

Source Tissue Comment Organism Textmining
amastigote
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 Leishmania mexicana
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promastigote
-
 Leishmania mexicana
-

Synonyms

Synonyms Comment Organism
LmxM11.1000
-
 Leishmania mexicana
PPDK
-
 Leishmania mexicana
pyruvate phosphate dikinase
-
 Leishmania mexicana

General Information

General Information Comment Organism
malfunction no difference in growth is observed between PPDK wild-type and null mutant parasite promastigotes, but the incorporation of precursors into storage compound mannogen changes in the mutant compared to wild-type Leishmania mexicana
metabolism the gluconeogenesis pathway in Leishmania parasites involves glycerol kinase, phosphoenolpyruvate carboxykinase, and pyruvate phosphate dikinase which allow the entry of glycerol, aspartate, and alanine into Leishmania mannogen, respectively. Contribution of these enzymes into gluconeogenesis differs between promastigotes and amastigotes, model of gluconeogenesis, overview Leishmania mexicana
physiological function the enzyme PPDK is involved in the entry of alanine into the essential gluconeogenesis pathway in amastigotes. The majority of alanine enters into the pathway via decarboxylation of pyruvate in promastigotes. Also L-lactate, an abundant glucogenic precursor in mammals, is used by Leishmania amastigotes to synthesize mannogen, entering the pathway through PPDK Leishmania mexicana