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Literature summary for 2.7.9.1 extracted from
- Phosphoenolpyruvate synthetase and pyruvate, phosphate dikinase of Thermoproteus tenax: key pieces in the puzzle of archaeal carbohydrate metabolism (2006), Mol. Microbiol., 60, 287-298.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | neither inhibition nor activation of anabolic PPDK activity observed, kinetics of catabolic pathway | Thermoproteus tenax |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Escherichia coli, strains DH5alpha and BL21-CodonPLUS(DE3)-RIL | Thermoproteus tenax |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | concentration 1 mM, 55°C, AMP-competitive inhibition as deduced from AMP saturation kinetics by using various ATP concentration ranging between 50-200 micromol | Thermoproteus tenax |  |
| D-glucose 1-phosphate | 72% activity sustained, effector concentration 5 mM, 55°C | Thermoproteus tenax | |
| additional information | inhibition by UDP and D-glucose 1-phosphate at rather high and unphysiological concentrations, no inhibitory effects by alpha-ketoglutarate (1 mM), potassium phosphate (5 mM), glyceraldehyde 3-phosphate (5 mM), 3-phosphoglycerate (5 mM), dihydroxyacetone phosphate (5 mM) | Thermoproteus tenax | |
| UDP | 55% activity of PPDK, effector concentration 5 mM, 55°C | Thermoproteus tenax | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | reactions follow classical Michaelis Menten kinetics for pyruvate and phosphate, sigmoidal saturation curve observed with ATP, neither inhibition nor activation of the anabolic PPDK activity observed | Thermoproteus tenax | |
| 0.02 | - |
AMP | 55°C | Thermoproteus tenax | |
| 0.08 | - |
diphosphate | 55°C | Thermoproteus tenax | |
| 0.5 | - |
phosphoenolpyruvate | 55°C | Thermoproteus tenax | |
| 0.8 | - |
pyruvate | 70°C | Thermoproteus tenax | |
| 3.5 | - |
diphosphate | 70°C | Thermoproteus tenax | |
| 8 | - |
ATP | KM/S50-value, 70°C | Thermoproteus tenax | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Thermoproteus tenax | |
| additional information | not depending on monovalent cations such as K+, Na+, NH4+ | Thermoproteus tenax |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
x * 100000, SDS-PAGE, homomeric dimer, calculated from theoretical weight and chromatography, more similar to bacterial PPDKs (dimer) than protists or plants (tetramer) | Thermoproteus tenax |
| 102300 | - |
theoretical weight calculated from sequence | Thermoproteus tenax |
| 250000 | - |
native protein, gelfiltration | Thermoproteus tenax |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina acetivorans | Q8TT31 | Euryarchaea, Archaeabacteria | - |
| Pyrobaculum aerophilum | Q8ZT84 | Crenarchaea, Archaeabacteria | - |
| Thermoproteus tenax | Q70WQ6 | the entire gene consists of 2748 bp, GC content of 52.6%, protein of 915 amino acids corresponding to a theoretical molecular mass of 102.3 kDa, sequence signatures compared to other PPDK genes; hyperthermophilic crenarchaeote, Archaeabacteria, mass culture of Thermoproteus strain DSM 2078 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein, heat precipitation at 90°C for 20 min, ion exchange chromatography | Thermoproteus tenax |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the maximal specific activity of the catabolic reaction is approximately 2fold higher if compared to the anabolic reaction, indicated by lower Km-values for reaction compounds of the catabolic reaction, kinetics at 55°C and 70°C shown, neither inhibition nor activation of anabolic PPDK activity observed | Thermoproteus tenax |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + phosphate | bidirectional activity, preference for catabolic reaction, DELTA G +9.9 kJ/mole | Thermoproteus tenax | AMP + phosphoenolpyruvate + diphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | x * 100000, SDS-PAGE, homomeric dimer, calculated from theoretical weight and chromatography, more similar to bacterial PPDKs (dimer) than protists or plants (tetramer) | Thermoproteus tenax |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PPDK | - |
Thermoproteus tenax |
| pyruvate, phoshphate dikinase | - |
Thermoproteus tenax |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | assay overview, kinetics at 55°C and 70°C, anabolic and catabolic direction | Thermoproteus tenax |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
ATP | 55°C, AMP saturation kinetics with ATP concentration of 50-200 micromol | Thermoproteus tenax | |
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